Swaczynová, Jana; Šíša, Miroslav; Hniličková, Jaroslava; Kohout, Ladislav; Strnad, Miroslav
POLISH JOURNAL OF CHEMISTRY
80 [4]:
629-635,
2006
Keywords:
Brassinosteroids; phytohormones; antibodies; structure-activity relationship
Abstract:
We have developed polyclonal antibodies against the brassinosteroid, 24-epicastasterone. Antiserum against this substance was produced by immunizing rabbits and mice with 24-epicastasterone O-(carboxymethyl)oxime (24-epiCS-CMO) conjugated with bovine-serum albumin (BSA). The use of the ELISA allowed detection over the range of 0.01 500 pmoles. Natural brassinosteroids (BRs) like brassinolide, and 24-epibrassinolide exhibited relatively high cross-reactivities but many other natural BRs were inactive.
IEB authors: Miroslav Strnad
Čeština
English