Wicker, T., Mayer, K.F.X., Gundlach, H., Martis, M., Steuernagel, B., Scholz, U., Šimková, H., Kubaláková, M., Choulet, F., Taudien, S., Platzer, M., Feuillet, C., Fahima, T., Budak, H., Doležel, J., Keller, B., Stein, N.
PLANT CELL
23:
1706-1718,
2011
Klíčová slova:
Abstrakt:
All six arms of the group 1 chromosomes of hexaploid wheat (Triticum aestivum) were sequenced with Roche/454 to 1.3- to
2.2-fold coverage and compared with similar data sets from the homoeologous chromosome 1H of barley (Hordeum
vulgare). Six to ten thousand gene sequences were sampled per chromosome. These were classified into genes that have
their closest homologs in the Triticeae group 1 syntenic region in Brachypodium, rice (Oryza sativa), and/or sorghum
(Sorghum bicolor) and genes that have their homologs elsewhere in these model grass genomes. Although the number of
syntenic genes was similar between the homologous groups, the amount of nonsyntenic genes was found to be extremely
diverse between wheat and barley and even between wheat subgenomes. Besides a small core group of genes that are
nonsyntenic in other grasses but conserved among Triticeae, we found thousands of genic sequences that are specific to
chromosomes of one single species or subgenome. By examining in detail 50 genes from chromosome 1H for which BAC
sequences were available, we found that many represent pseudogenes that resulted from transposable element activity and
double-strand break repair. Thus, Triticeae seem to accumulate nonsyntenic genes frequently. Since many of them are likely
to be pseudogenes, total gene numbers in Triticeae are prone to pronounced overestimates.
Fulltext: kontaktujte autory z ÚEB
Autoři z ÚEB: Jaroslav Doležel,
Marie Kubaláková,
Hana Šimková
Čeština
English