Curriculum vitae:
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1980-1986
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University of Palacký, Faculty of Natural Sciences, Olomouc, Czech Republic
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1986 – 1989
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Institute of Animal Physiology and Genetics, Czechoslovak Academy of Sciences,
Prague, Czech Republic, degree CSc. (eq. PhD), PhD Thesis: Incidence of plasmids in Ruminal bacteria
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1989 – present
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Institute of Animal Physiology and Genetics, Academy of Sciences of Czech
Republic, Laboratory of anaerobic mikrobiology, scientist, member of staff
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Specialization:
I am fully interested in microbiology of mammal digestive tract with special emphasis on microbes of herbivorous ruminants. Among microorganisms occupying rumen ecosystem, which are bacteria, archaea, protozoa and fungi, I pay special attention to gut anaerobic fungi. Their isolation,characterisation and biotechnological application is my main research activity. The recent interest in microbial diversity of biogas plants is natural consequence of our effort to apply anaerobic microbes of gastrointestinal tract in biotechnology.
Societies:
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Czechoslovak society for microbiology
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Internship abroad:
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1993
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The Rowett Research Institute, Aberdeen, Scotland, U.K.
The study of plasmids in ruminal strains of Selenomonas ruminantium
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1996
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The Auburn University, U.S.A.
The Study of anaerobic microbial population in caecum of chicken
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2005
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Hokkaido University, Sapporo, Japan
Study of new phylogenetic group of Selenomonas ruminantium
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Publications:
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2004
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Fliegerová, K., Hodrová, B., Voigt, K. 2004. Classical and molecular approaches as a powerful tool for the characterization of rumen polycentric fungi. Folia Microbiologica 49: 157-164
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2006
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Fliegerová, K., Mrázek, J., Voigt, K. 2006. Differentiation of Anaerobic Polycentric Fungi by rDNA PCR-RFLP. Folia Microbiologica, 51: 273-277
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K.Fliegerová, K.Voigt. 2006. Molecular phylogeny of the Neocallimastigales (Mycota)
based on ribosomal RNA genes: exploring the root of the fungal kingdom. Reproduction Nutrition Development 46: S14-S15.
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2008
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K.Fliegerová, K.Hoffmann, J.Mrázek, K.Voigt. 2008. The design of oligonucleotide primers for the universal amplification of the N-acetylglucosaminidase gene (nag1) in
Chytridiomycetes with emphasis on the anaerobic Neocallimastigales. Folia Microbiologica 53: 209-213
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Z.Novotná, K.Fliegerová, J.Šimůnek. 2008. Characterization of chitinases of anaerobic
polycentric rumen fungi . Folia Microbiologica 53: 241-245
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I.Koppová, Z.Novotná, L.Štrosová, K. Fliegerová. 2008. Analysis of fatty acid composition of anaerobic rumen fungi. Folia Microbiologica 53: 217-220
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J. Mrázek, L. Štrosová, K .Fliegerová, T. Kott, J. Kopečný. 2008. Diversity of insect intestinal microflora. Folia Microbiologica 53: 229-233.
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J. Kopečný, J. Mrázek, K.Fliegerová, P. Fruhauf, L. Tuckova. 2008. The intestinal microflora of childhood patients with indicated celiac disease. Folia Microbiologica 53:214-216.
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2010
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K. Fliegerová, J.Mrázek,K.Hoffmann, J. Zábranská, K.Voigt. 2010. Diversity of anaerobic fungi within cow manure determined by ITS1 analysis. Folia Microbiologica, 55: 319-325.
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Z. Novotná, J. Procházka , J. Šimůnek , K. Fliegerová . 2010. Xylanases of anaerobic fungus Anaeromyces mucronatus. Folia Microbiologica, 55: 363-367.
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J. Mrázek, I. Koppová, J. Kopečný, J. Šimůnek, K. Fliegerová. 2010. PCR-DGGE-based study of fecal microbial stability during the long-term chitosan supplementation of human. Folia Microbiologica, 55: 352-358.
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Griffith GW, Baker S, Fliegerova K, Liggenstoffer A, van der Giezen M, Voigt K, Beaks G. 2010. Anaerobic fungi: Neocallimastigomycota. IMA Fungus 1, 181-185
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M. Eckart, K. Fliegerová, K. Hoffmann, K. Voigt. Molecular identification of anaerobic rumen fungi. In: Molecular Identification of Fungi, pp. 297 – 313, Y. Gherbawy, K. Voigt (Eds.), Springer-Verlag Berlin Heidelberg, 2010.
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| 2011 |
Fliegerova, K. , Mrazek, J., Kajan. M., Podmirseg, S.M., Insam, H. 2012.The Effect of maize silage as co-substrate for swine manure on the bacterial community structure in biogas plants. Folia Microbiologica, 57: 281-284.
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Fliegerová, K., Procházka, J., Mrázek, J., Novotná, Z., Štrosová, L., Dohanyos, M. Biogas and rumen fungi. In: Litonjua et al. editors. Biogas: production, consumption and applications. Hauppauge, New York, USA: Nova Science Publisher Inc; 2012; pp. 161-179, ISBN 978-1-61470-973-2 (Hardcover book), ISBN 978-1-62100-023-5 (e-book)
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Rosero, J.A., Strosova, L., Mrazek, J., Fliegerova, K., Kopečny J. 2012. PCR detection of uncultured rumen bacteria. Folia Microbiologica, 57: 325-330.
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Schoch, C. L., Seifert, K.A., Huhndorf, S., Robert, V., Spouge,J.L., Levesque,C.A.,Chen,W.,and Fungal Barcoding Consortium. 2012. Nuclear ribosomal internal transcribed spacer (ITS) region as a universal DNA barcode marker for Fungi. PNAS 109 (16), 6241-6246. doi: 10.1073/pnas.1117018109
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Fliegerova, K., Mrazek, J., Voigt, K. 2012. Gut fungi: Classification, evolution, life style and application. In: Fungi: Types, environmental impact and role in desease. Editors: A.P. Silva and M. Sol, pp. 3-20. Hauppauge, New York, USA: Nova Science Publisher Inc; ISBN 978-1-61942-671-9 (Hardcover book), 978-1-61942-685-6 (e-book)
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Prochazka, J., Mrazek, J., Štrosová, L., Fliegerová, K., Zábranská, J., Dohányos, M. 2012. Enhanced biogas yield from energy crops with rumen anaerobic fungi. Eng. Life Sci. 12, 3, 343-351.
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| 2014 |
Fliegerova, K., Tapio, I., Bonin, A., Mrazek, J., Callegari, M., Bani, P., Bayat, A., Vilkki, J., Kopečný, J., Shingfield, K.J., Boyer, F., Coissac, E., Taberlet, P., Wallace, R.J. 2014. Effect of DNA extraction and sample preservation method on rumen bacterial population, Anaerobe, 29, 80-84. doi.org/10.1016/j.anaerobe.2013.09.015;
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Leis S, Dresch P, Peintner U, Fliegerová K, Sandbichler AM, Insam H, Podmirseg SM. 2014. Finding a robust strain for biomethanation: Anaerobic fungi (Neocallimastigomycota) from the Alpine ibex (Capra ibex) and their associated methanogens. 2014, 29, Anaerobe. 2014 Oct;29:34-43. doi: 10.1016/j.anaerobe.2013.12.002.
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Schoch, CL, Robbertse, B, Robert, V, Vu, D, Cardinali, G, et al. Finding needles in haystacks: linking scientific names, reference specimens and molecular data for Fungi. Database-The Journal Of Biological Databases And Curation, Article Number: bau061, DOI: 10.1093/database/bau061 (2014)
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Gruninger, RJ, Anil K. Puniya, AK, Callaghan, TM, Edwards, JE, Youssef, N, Dagar, SS, Fliegerova, K, Griffith, GW, Forster, R, Tsang, A, McAllister, T, Elshahed, MS. 2014. Anaerobic Fungi (Phylum Neocallimastigomycota):Advances in understanding of their taxonomy, life cycle, ecology, role, and biotechnological potential. FEMS Microbiol. Ecol. 90,1,1-17. DOI: 10.1111/1574-6941.12383
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