Blagoeva, Elitsa; Malbeck, Jiří; Gaudinová, Alena; Vaněk, Tomáš; Vaňková, Radomíra
BIOTECHNOLOGY LETTERS
25 [6]:
469-472,
2003
Keywords:
alginate; cytokinins; plant cell immobilization
Abstract:
Dynamics of the response of tobacco cells (line BY-2) to exogenous cytokinin, N6-benzyladenine, and cyclin-dependent kinase inhibitor, roscovitine, was followed using alginate-immobilized cells packed into a column. N6-Benzyladenine (1.25 μM) increased the synthesis of the physiologically-active endogenous cytokinin, isopentenyladenosine, in the effluent up to 0.1 nM. Simultaneously, conversion of the excess of endogenous cytokinins to biologically inactive derivatives of cis-zeatin occurred, up to 0.8 nM. Roscovitine (50 μM) further increased cis-cytokinins, up to 2.2 nM.
IEB authors: Alena Gaudinová,
Jiří Malbeck,
Tomáš Vaněk,
Radomíra Vanková
Čeština
English