The large size and polyploid nature of the nuclear genome of bread wheat pose a great challenge for the understanding of complete genome information, genome sequencing and gene discovery. This task could be simplified by fractionating the genome into small and defined parts. Here we propose to create two subgenomic BAC libraries specific for short and long arm of wheat chromosome 3D. Their creation will mark the completion of BAC resources for homeologous group 3, which has been selected for the pilot phase of the wheat genome sequencing project.
