Curriculum vitae:
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1976-1982
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Veterinary and Pharmaceutical University Brno
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1983-1989
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Specialist, Institute of Animal Physiology and Genetics, AS CR, v.v.i., Prague
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1989
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PhD Thesis: Manipulation of the Rumen Fermentation
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1989-2000
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Research worker, Institute of Animal Physiology and Genetics, AS CR, v.v.i., Prague
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2000-present
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Independent research worker, Institute of Animal Physiology and Genetics, AS CR, v.v.i., Prague
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Specialization:
Microbiology and biochemistry of anaerobic bacteria from the animal and human digestive tract, chitin enzyme degradation, chitinases, chitinolytic bacteria of the human and animal gastrointestinal tract.
Awards:
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2009
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3rd Award Prize for poster presentation, conference EUCHIS 2009, Venice
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2011
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3rd Award Prize for poster presentation, conference EUCHIS 2011, St. Petersburg
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Internship abroad:
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1987
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Institute of Animal Physiology and biochemistry, Lviv, Ukraine
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1988
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Institute of Animal Physiology, Alma-Ata, Kazakhstan
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1989
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Rowett Research Institute, Aberdeen, Scotland, U.K.
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1989
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The Kielanowski Institute of Animal Physiology and Nutrition, Jablona, Poland
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Publications:
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2007
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Kopečný J., Šimůnek J.: Chitosan application in food preparation and digestion. Adv. Chitin Sci. X, Senel S., Varum KM, Sumnu MM, Hincal AA, Eds., alp Offset, Ankara, pp. 467-473
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2008
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Belzecki G., Miltko R., Michalowski T., Šimůnek J., Kopečný J.: chitinolytic aktivity of the sheep rumen ciliate Diploplastron affine. Folia Microbiol. 53: 201-203
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2008
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Šimůnek J., Tishchenko G., Koppová I.: Chitinolytic activities of Clostridium sp. JM2 isolated from stool of human administered per orally by chitosan, Folia Microbiologica 53:249-254
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2010
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Lukáš F., Šimůnek J., Mrázek J., Kopečný J.: PCR-DGGE Analysis of bacterial population attached to the bovine rumen wall. Folia Microbiol. 55: 345-348
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2010
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Tishchenko G., Koppová I.: Chitinolytic activities of Clostridium sp. JM2 isolated from stool of human administered per orally by chitosan, Folia Microbiologica 53:249-254
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2010
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Šimůnek J., Koppová I., Lukáš F., Tishchenko G., Belzecki G.: The antimicrobial action of low-molar-mass chitosan, chitosan derivatives and chitooligosaccharides on bifidobacteria. Folia Microbiol. 55: 379-382
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2010
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Tishchenko G., Koppová I., Šimůnek J., Dohnálek J.: Extracellular comlex of chitinolytic enzymes of Clostridium paraputrificum strain J4 separated by membrane ultrafiltration, Folia Microbiologica 55:386-389
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2010
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Mrázek J., Koppová I., Kopečný J., Šimůnek J., Fliegerová K: PCR-DGGE based study of fecal microbial stability during the long-term chitosan supplementation of human. Folia Microbiologica 55:352-358
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2011
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Dušková J., Tishchenko G., Ponomareva E., Šimůnek J., Koppová I., Skálová T., Šťěpánková A., Hašek J., Dohnálek J.: Chitinolytic enzymes from bacterium inhabiting human gastrointestinal tract – critical parameters of protein isolation from anaerobic culture. Acta Biochima Polonica 58:261-263
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2011
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Tishchenko G., Šimůnek J., Brus J., Netopilík M., Pekárek M., Walterová Z., Koppová I., Lenfeld J.: Low-molecular-weight-chitosans: Preparation and characterization. Carbohydrate Polymers, 86:1077-1081
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2011
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Tishchenko G., Šimůnek J., Bartoňová H., Dušková J., Dohnálek J.: Sample preparation in separation of the extracellular chitinolytic enzymes of the human intestinal bacterium Clostridium paraputrificum J4 from the culture fluids. J. Chromatography B, 879: 2175-2178
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