Genetic blueprint of bread wheat genome unveiled

The genetic blueprint is an invaluable resource to plant science researchers and breeders. For the first time, they have at their disposal a set of tools enabling them to rapidly locate specific genes on individual wheat chromosomes throughout the genome. Jorge Dubcovsky, Professor at the University of California Davis, USA, says that these results “have been a fantastic resource for our laboratory. The development of genome specific primers, which used to take several weeks of work, can now be done in hours. Mapping of any sequence to the specific chromosome arm can now be done in silico in minutes. In addition to the acceleration of day to day work in wheat genetics, this resource has made possible analyses and discoveries at the genome level that were not possible before.“

 

 

The draft sequence is a major landmark towards obtaining a complete reference sequence of the hexaploid bread wheat genome, the ultimate aim of the International Wheat Genome Sequencing Consortium. In the same issue of Science, another article presents the reference sequence for the first of the 21 wheat chromosomes. The team of Professor Doležel participated also on this project, which was led by The French National Institute for Agricultural Research. The reference sequence of the largest wheat chromosome 3B, with the size equivalent to one third of the human genome, establishes a proof of concept for Doležel’s chromosome-based strategy and a template for sequencing the remaining chromosomes. As of today, researchers in the IWGSC estimate that the full genome sequence will be available within three years.

 

“With the draft gene sequence for each of the bread wheat chromosome and the first reference sequence of chromosome 3B, we have reached a great milestone in our roadmap,” said Catherine Feuillet, IWGSC co-chair. “We know now the way forward to obtain a reference sequence for the 20 remaining chromosomes and we hopefully will be able to find the resources to achieve this in the next three years.”

 

The team of Professor Jaroslav Doležel developed the chromosome-based strategy to unlock the complex genome of wheat, which is about six times larger the human genome. The team established a unique set of tools for purification of individual chromosomes and they produce chromosome-specific molecular resources for members of IWGSC. This facilitates sequencing the wheat genome following the chromosome by chromosome strategy. Thanks to this, the Czech scientists participate on sequencing all 21 chromosomes of wheat. „Both publications and the impact these advances makes are a terrific reward for many years of hard and systematic work of our team and a great motivation to continue. We are responsible for sequencing three chromosomes and collaborate with leading laboratories in USA, France, Germany and Japan, among other. At the same time, we have been using the new knowledge and tools to clone important wheat genes,“ said Jaroslav Doležel.

 

 

With a chromosome-based full sequence in hand, plant breeders will have high quality tools at their disposal to accelerate breeding programs and to identify how genes control complex traits such as yield, grain quality, disease, pest resistance, or abiotic stress tolerance. They will be able to produce a new generation of wheat varieties with higher yields and improved sustainability to meet the demands of a growing world population in a changing environment. 

 

The draft sequence is already providing new insights into the history and evolution of the wheat genome and genes involved in grain development, as exemplified in two additional publications appearing in the same issue of Science.

 

Wheat is a major dietary component for many populations across the world. Grown on more land than any other crop, more than 215 million hectares of wheat are harvested annually to generate a world production of almost 700 million tons, making it the third most produced cereal after maize and rice. It is the leading source of vegetable protein in human food, having a higher protein content than either maize or rice. The wheat plant is highly versatile due to its ability to grow in a wide range of environments. Wheat grain is easily stored and can be converted readily into flour for making numerous varieties of high quality edible food.

 

About the IWGSC:

The IWGSC, with more than 1,000 members in 57 countries, is an international, collaborative consortium, established in 2005 by a group of wheat growers, plant scientists, and public and private breeders. The goal of the IWGSC is to make a high quality genome sequence of bread wheat publicly available, in order to lay a foundation for basic research that will enable breeders to develop improved varieties.

 

More information can be found at http://olomouc.ueb.cas.cz and www.wheatgenome.org

 

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