Centre of Plant Structural and
Functional Genomics
of the Institute of Experimental Botany AS CR

With the aim to facilitate high-throughput genome profiling and genetic mapping within the complex, we have developed a Diversity Arrays Technology (DArT) array for five important species: F. pratensis, F. arundinacea, F. glaucescens, L. perenne and L. multiflorum. The DArTFest array contains 7680 probes derived from methyl-filtered genomic representations.

The potential use of the DArTFest array:

• Analysis of genetic diversity
• Genotyping
• Analysis of genomic constitution of hybrids
• Genetic and physical mapping
• Simultaneous marker-assisted selection for multiple traits or specific genome regions

The databasis for DArTFest array is freely available (http://bioinf.scri.ac.uk/germinate_grasses).

Kopecký, D., Bartoš, J., Lukaszewski, A.J., Baird, J.H., Černoch, V., Kölliker, R., Rognli, O.A., Blois, H., Caig, V., Lübberstedt, T., Studer, B., Shaw, B., Doležel, J. and Kilian, A. (2009). Development and mapping of DArT markers within the Festuca – Lolium complex. BMC Genomics 10:473.

Kopecký, D., Bartoš, J., Christelová, P.,Černoch, V., Kilian, A., Doležel, J. (2011). Genomic constitution of Festuca × Lolium hybrids revealed by the DArTFest array. Theoretical and Applied Genetics 122:355–363.

Bartoš, J., Sandve, S.R., Kölliker, R., Kopecký, D., Stočes, Š., Christelová, P., Østrem, L., Larsen, A., Kilian, A., Rognli, O.A. and Doležel, J. (2011). Genetic mapping of DArT markers in the Festuca–Lolium complex and their use in freezing tolerance association analysis. Theoretical and Applied Genetics 122:1133–1147.

Baird, J., Kopecký, D., Lukaszewski, A.J., Green, R.L., Bartoš, J. and Doležel, J. (2012). Genetic Diversity of Turf-Type Tall Fescue Using Diversity Arrays Technology. Crop Science doi: 10.2135/cropsci2011.02.0106.