Trifunović M. *, Motyka V. *, Cingel A., Subotić A., Jevremović S., Petrić M., Holík J., Malbeck J., Dobrev P.I., Dragićević I.Č.
PLANT CELL, TISSUE AND ORGAN CULTURE
120:
767-777,
2015
Keywords:
Centaurium erythraea Rafn., cytokinin, cytokinin oxidase/dehydrogenase, AtCKX genes, hormone metabolism, plant development
Abstract:
The plant hormones cytokinins (CKs) regulate
a number of physiological processes. Their homeostasis is
controlled by the rate of de novo synthesis and the rate of
catabolism. The aim of this work was to analyze the content
of total as well as individual groups of endogenous
CKs in AtCKX1 and AtCKX2-overexpressing centaury
(Centaurium erythraea Rafn.) plants grown in vitro.
Transgenic CKX plants represent a suitable model system
for studying physiological and morphological processes
controlled by CKs. In this work we clearly demonstrate a
significant effect of AtCKX transgenes on CK metabolism
in transgenic centaury plants. However, shoots and roots of
only one AtCKX1 line and three AtCKX2 lines with a
significant reduction of bioactive CKs were obtained. We
also show that changes in the CKs metabolism considerably
affected endogenous indole-3-acetic acid (IAA) levels
in plant tissues. All analyzed transgenic AtCKX centaury
lines exhibited decreased amount of endogenous IAA in
shoots as well as in roots. Consequently, the IAA/bioactive
CK forms ratios showed a significant variation in the
shoots and roots of all analyzed AtCKX centaury
transformants.
Fulltext: contact IEB authors
IEB authors: Petre I. Dobrev,
Josef Holík,
Jiří Malbeck,
Václav Motyka
Čeština
English