Dierking, R., Azhaguvel, P., Kallenbach, R., Saha, M., Bouton, J., Chekhovskiy, K., Kopecký, D., Hopkins, A.
THE PLANT GENOME
8:
1-18,
2015
Klíčová slova:
Abstrakt:
Temperate grasses belonging to the Festuca–Lolium complex are
important throughout the world in pasture and grassland agriculture.
Tall fescue (Festuca arundinacea Schreb.) is the predominant
species in the United States, covering approximately 15 million
ha. Tall fescue has distinctive morphotypes, two of which are
Continental (summer active) and Mediterranean (summer semidormant).
This is the first report of a linkage map created for
Mediterranean tall fescue, while updating the Continental map
with additional simple sequence repeat and sequence-tagged
site markers. Additionally,
this is the first time that diversity arrays
technology (DArT) markers were used in the construction of a tall
fescue map. The male parent (Continental), R43-64, map consisted
of 594 markers arranged in 22 linkage groups (LGs) and
covered a total of 1577 cM. The female parent (Mediterranean),
103-2, map was shorter (1258 cM) and consisted of only 208
markers arranged in 29 LGs. Marker densities for R43-64 and
103-2 were 2.65 and 6.08 cM per marker, respectively. When
compared with the other Poaceae species, meadow fescue (F.
pratensis Huds.), annual ryegrass (L. multiflorum Lam.), perennial
ryegrass (L. perenne L.), Brachypodium distachyon (L.) Beauv., and
barley (Hordeum vulgare L.), a total of 171 and 98 orthologous or
homologous sequences, identified by DArT analysis, were identified
in R43-64 and 103-2, respectively. By using genomic in situ
hybridization, we aimed to identify potential progenitors of both
morphotypes. However, no clear conclusion on genomic constitution
was reached. These maps will aid in the search for quantitative
trait loci of various traits as well as help define and distinguish
genetic differences between the two morphotypes.
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