Tomáš Špringer, MSc.

Research interests:

Study of nucleic acids and their analogues significant in therapies based on suppression of genetic expression.

Development of microfluidics for SPR biosensors.

Application of gold nanoparticles for ultra-sensitive SPR biosensors.

Surface plasmon resonance (SPR) biosensors.

Education:

2006 - today: PhD study, Charles University, Faculty of Mathematics and Physics (Biophysics) at the Institute of Photonics and Electronics, dpt. of Optical Sensors

Thesis: In situ study of interactions of nucleic acids significant in genetic expression and therapy based on its suppression
Supervisor: Prof. Josef Stepanek, PhD.

2001 - 2006: Master of Science, Charles University, Faculty of Mathematics and Physics (Biophysics)

Thesis: Surface-enhanced photophysical processes of organic molecules in metal nanoparticles
Supervisor: Doc. RNDr. Marek Procházka, Dr.

Awards:

2^nd poster award, Europt(r)ode X 2010, Prague.

Publications:

Book chapters:

1. P. Adam, M. Piliarik, H. Šípová, T. Špringer, M. Vala, J. Homola: Surface Plasmons for Biodetection, in Photonic Sensing: Principles and Applications for Safety and Security Monitoring, editors W. J. Bock, J., X. Gaozhi, John Wiley & Sons, 2012, 1-58.

Journal papers:

1. T. Špringer, M. L. Ermini, B. Špačková, J. Jabloňků, J. Homola: Enhancing sensitivity of SPR biosensors by functionalized gold nanoparticles: size matters, Analytical Chemistry, 86, 10350−10356 (2014)
2. H. Šípová, T. Špringer, D. Rejman, O. Šimák, M. Petrová, P. Novák, Š. Rosenbergová, O. Páv, R. Liboska, I. Barvík, J. Štěpánek, I. Rosenberg, J. Homola: 5’-O-Methylphosphonate nucleic acids (MEPNA) – new modified DNAs that increase the E. coli RNase H cleavage rate of hybrid duplexes, Nucleic Acids Research, 42, 5378–5389 (2014). link
3. T. Špringer, M. Bocková, J. Homola: Label-Free Biosensing in Complex Media: A Referencing Approach.  Analytical Chemistry, 85 (2013), 5637–5640. link
4. T. Špringer, J. Homola: Biofunctionalized gold nanoparticles for SPR-biosensor-based detection of CEA in blood plasma. Analytical and Bioanalytical Chemistry, 404 (2012), 2869-2875. link
5. H. Šípová, T. Špringer, J. Homola: Streptavidin-enhanced assay for sensitive and specific detection of single nucleotide polymorphism in TP53. Analytical and Bioanalytical Chemistry, 399 (2011), 2343-2350. link
6. T. Špringer, H. Šípová, H. Vaisocherová, J. Štepánek, J. Homola: Shielding effect of monovalent and divalent cations on solid-phase DNA hybridization: surface plasmon resonance study. Nucleic Acids Research, 38 (2010), 7343-7351. link
7. T. Špringer, M. Piliarik, J. Homola: Surface plasmon resonance sensor with dispersionless microfluidics for direct detection of nucleic acids at the low femtomole level. Sensors and Actuators B - Chemical, 145 (2010), 588-591. link
8. T. Špringer, M. Piliarik, J. Homola: Real-time monitoring of biomolecular interactions in blood plasma using a surface plasmon resonance biosensor. Analytical and Bioanalytical Chemistry, 398 (2010),1955-1961. link
9. H. Vaisocherová, J. Snášel, T. Špringer, H. Šípová, I.  Rosenberg, J. Štěpánek, J. Homola: Surface plasmon resonance study on HIV-1 integrase strand transfer activity. Analytical and Bioanalytical Chemistry, 393 (2009), 1165–1172. link

Conference papers:

1. T. Špringer, M. L. Ermini, P. Kvasnička, B. Špačková, J. Homola: Size effect of neutravidin-coated gold nanoparticles on the enhanced SPR sensor response in the detection of carcinoembryonic antigen. Advances in Biodetection and Biosensors 2014, Berlin.
2. T. Špringer, J. Homola: Functionalized nanoparticles for the amplification of sensor response in SPR biosensors. Europt(r)ode XI 2012, Barcelona.
3. P. Kvasnička, T. Špringer, J. Homola: Single-DNA binding event detection in amplified micro-SPR surface plasmon resonance sensor. Europt(r)ode XI 2012, Barcelona.
4. T. Špringer, M. Piliarik, J. Homola: Real-time monitoring of biomolecular interactions in blood plasma using surface plasmon resonance sensor. Europt(r)ode X 2010, Prague.
5. H. Šípová, T. Špringer, J. Homola: Streptavidin-enhanced assay for sensitive and specific detection of single nucleotide polymorphism in TP53. Europt(r)ode X 2010, Prague.
6. M. Vala, H. Šípová, T. Špringer, K. Chadt, M. Piliarik, J. Homola: Compact multi-channel surface plasmon resonance sensor for rapid detection of bacterial pathogens. Europt(r)ode X 2010, Prague.