Nevrtalova, E., Baloun, J., Hudzieczek, V., Cegan, R., Vyskot, B., Doležel, J., Šafář, J., Milde, D., Hobza, R.
PROTOPLASMA
251:
1427-1439,
2014
Klíčová slova:
Copper, Gene duplication, Metallothionein, Heavymetal tolerance, Silene vulgaris
Abstrakt:
Metallothioneins (MTs) were identified as important
players in metal metabolism. MT3 gene presents a key
metallothionein controlling copper homeostasis in plants. We
have selected one cupricolous and one non-cupricolous ecotype
to isolate and analyse the MT3 gene in Silene vulgaris.
For expression data comparison, we have also included other
metal-tolerant ecotypes. Based on a S. vulgaris BAC library
screening, we have identified and sequenced a genomic clone
containing MT3 gene (SvMT3). We found that SvMT3 gene
has been locally duplicated in a tandem arrangement. Expression
analysis and complementation studies using yeast mutants
showed that both copies of the SvMT3 gene were functional.
Moreover, we examined the expression of MT3 gene(s)
in selected ecotypes under different copper treatments to show
the tissue-specific expression response to copper stress. We
demonstrated that higher copper concentrations specifically
affected MT3 expression among ecotypes. Our analysis shows
that MT3a has similar expression pattern in cupricolous
ecotypes while MT3b has common expression features shared
by allmetallophyte S. vulgaris ecotypes. Our data indicate that
down-regulation of MT3b root expression in higher copper
concentrations is associated with copper stress. We propose
that there might be a specific regulation of SvMT3s transcription
depending on the type of heavy metal tolerance.
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Autoři z ÚEB: Jaroslav Doležel,
Roman Hobza,
Jan Šafář
Čeština
English