Hřibová, E., Christelová, P., Roux, N., Doležel, J.
ACTA HORTICULTURAE
986:
241-246,
2013
Klíčová slova:
fragment analysis, genotyping platform, microsatellite markers, Musa, Musa Genotyping Centre, nuclear genome
Abstrakt:
Banana and plantain (Musa spp.) are seed-sterile vegetatively propagated
crops, which originated as intraspecific hybrids of Musa acuminata and interspecific
hybrids between M. acuminata and Musa balbisiana. The rich genetic diversity of
this crop is, however, endangered by diseases, adverse environmental conditions and
changing farming practices. To set up an efficient and effective strategy for breeding
improved banana cultivars and support the choice of crossing-parents, a solid
understanding of the genetic diversity of available resources is needed, underlining
the need for characterization and conservation of this genetic diversity. With the aim
to provide a simple and robust approach for genotyping in Musa, we have developed
an optimized genotyping platform using SSR markers. The genotyping system is
based on 19 microsatellite loci, originally developed by CIRAD, which are scored
using fluorescently labeled primers and high-throughput capillary electrophoresis
separation with high resolution. The knowledge gained during the course of this
study is used by the Musa Genotyping Centre, which serves the Musa research and
breeding community by determining DNA ploidy and SSR profiles of unknown
samples. The Centre operates on a cost-recovery basis and guarantees that all
analyses are performed under strictly controlled conditions, enabling direct
comparison of various accessions from different parts of the world.
Fulltext: kontaktujte autory z ÚEB
Autoři z ÚEB: Pavla Christelová,
Jaroslav Doležel,
Eva Hřibová
Čeština
English