Trifunović M., Cingel A., Simonović A., Jevremović S., Petrić M., Dragićević I.Č., Motyka V., Dobrev P.I., Zahajská L., Subotić A.
PLANT CELL, TISSUE AND ORGAN CULTURE
115:
139-150,
2013
Keywords:
Centaurium erythraea Rafn., Agrobacterium tumefaciens, genetic transformation, qRT-PCR, AtCKX genes, CKX activity
Abstract:
Cytokinin oxidase/dehydrogenase (CKX) is the
only known enzyme involved in cytokinin catabolism.
Genes coding for two Arabidopsis CKX isoforms, AtCKX1
and AtCKX2, were introduced separately into a binary
cloning vector, immobilized into Agrobacterium tumefaciens
strain GV3101, and introduced into root explants of
centaury (Centaurium erythraea Rafn.). The integration of
each transgene was confirmed by genomic PCR. Of the
total transformed explants, 30 and 28.2 % of the transformants
carried AtCKX1 and AtCKX2 transgenes, respectively.
Of these transformants, 50 % exhibited expression
of the AtCKX1 transgene, while 64 % of transformants
exhibited expression of the AtCKX2 transgene. For all
analysed AtCKX transgenic centaury lines, as well as for
untransformed control plants, CKX activity was higher in
roots than in shoots. Expression of AtCKX in most transgenic
lines contributed to enhanced levels of CKX activity
in root tissues; whereas, only a few lines demonstrated
increased CKX activity in shoot tissues compared to those
of control plants. Moreover, overexpression of AtCKX
resulted in reduced morphogenetic potential in transgenic
plants, but did not significantly affect biomass production
in comparison to untransformed control plants.
Fulltext: contact IEB authors
IEB authors: Petre I. Dobrev,
Václav Motyka,
Lenka Zahajská
Čeština
English