Steflova, P., Hobza, R., Vyskot, B., Kejnovsky, E.
CYTOGENETIC AND GENOME RESEARCH
142:
59-65,
2014
Klíčová slova:
Chloroplast DNA, Rumex acetosa, Sex chromosomes, Silene latifolia
Abstrakt:
Chloroplast DNA (cpDNA) sequences are often found in
plant nuclear genomes, but patterns of their chromosomal
distribution are not fully understood. The distribution of
cpDNA on the sex chromosomes can only be studied in
dioecious plant species possessing heteromorphic sex chromosomes.
We reconstructed the whole chloroplast genome
of Rumex acetosa (sorrel, XY 1 Y 2 system) from next generation
sequencing data. We systematically mapped the chromosomal
localization of various regions of cpDNA in R. acetosa
and in Silene latifolia (white campion, XY system) using fluorescence
in situ hybridization. We found that cpDNA was accumulated
on the Y chromosomes of both studied species.
In R. acetosa , the entire Y chromosome gathered all parts of
cpDNA equally. On the contrary, in S. latifolia , the majority of
the cpDNA, corresponding to the single copy regions, was
localized in the centromere of the Y chromosome, while the
inverted repeat region was present also in other loci. We
found a stronger accumulation of cpDNA on the more degenerated
Y 1 and Y 2 chromosomes of R. acetosa than in evolutionary
younger S. latifolia Y chromosome. Our data
stressed the prominent role of the Y chromosome centromere
in cpDNA accumulation.
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