Dwivedi S., Vaňková R., Motyka V., Herrera C., Žižková E., Auer C.
PLANT GROWTH REGULATION
61:
231-242,
2010
Klíčová slova:
cytokinin metabolism, N-glucosyltransferase, GRAM domain, aromatic cytokinins, roscovitine
Abstrakt:
Cytokinin analogue roscovitine exhibits a strong
inhibitory effect on cytokinin N-glucosylation, one of the
most important pathways of cytokinin inactivation in plants.
Roscovitine-resistant mutant. (ror-1) was isolated using TDNA
tagged lines of Arabidopsis thaliana (L.) Heynh in
order to find a gene putatively involved in cytokinin N-glucosylation.
The amount of cytokinin N-glucosides of transzeatin-
and isopentenyladenine-type was elevated by 20% in
ror-1 mutant compared to WT. The cytokinin oxidase/
dehydrogenase activity exhibited a mild elevation in ror-1
compared to WT in basal media. Additionally, ror-1 plants
showed slightly enhanced resistance to exogenously supplied
aromatic cytokinins (benzyladenine). Incubation with
exogenous cytokinin (5 lM BA for 24 h) resulted in significant
up-regulation of ROR-1 gene expression in ror-1
mutant. In silico analysis showed that ROR-1 gene encoded
for a protein consisting of GRAM (Glycosyltransferases
Rab-like GTPase activators and Myotubularins) and C2
domains. Here, we report on the role of ROR-1 gene in
metabolism of bioactive cytokinins in the plants.
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Autoři z ÚEB: Václav Motyka,
Radomíra Vanková,
Eva Žižková
Čeština
English