RNDr. Hana Lísalová , Ph.D. (Vaisocherová)

Profesní činnost:

2007 – 2008

Postdoctoral Research Associate, Department of Chemical Engineering at the University of Washington, Seattle.
Principal Investigator: Prof. Shaoyi Jiang

PROJECTS:

• Protein array development for cancer biomarker discovery and validation by means of label-free surface plasmon resonance technique. Collaboration with the Fred Hutchinson Cancer Research Center, Public Health Sciences Division, Seattle.
  • Developed a new label-free immunosensor for screening of cancer and normal human sera for presence of ALCAM with sensitivity comparable to conventional ELISA.
  • Antibodies to several candidate protein markers were selected with highest sensitivity and specificity to target detection from human plasma and cell lysate.
• Detection of various analytes (viruses, toxins) in complex matrices by SPR biosensor. Collaboration with Dr. Stacey Etheridge at the Food and Drug Administration, College Park.
  • Developed assay for sensitive detection of tetrodotoxin (TTX) in puffer fish matrix based on inhibition assay format.
  • Provided research advice to collaborators to successful validation of the developed TTX assay in inter-laboratory study.
• Characterization and functionalization of novel non-fouling biomaterials.
  • Optimized method to rapid and sensitive zwitterionic polymer characterization by combination of AFM and SPR spectroscopy.
  • Characterized super nonfouling surfaces in terms of non-specific binding from complex media including 100% blood plasma.
  • Developed a procedure to effective functionalization of novel zwitterionic polymer surfaces for biosensor and biomedical applications.

2003 –2007

Research Assistant, Institute of Photonics and Electronics of Academy of Sciences of the Czech Republic;
Principal Investigator : Assoc. Prof. Jiri Homola, Ph.D., DSc.

Investigation of immobilization methods for functionalization of immunosensors and protein/DNA/RNA chips.

• Developed a highly sensitive and specific sensor to detection of antibody against Epstein-Barr virus in human serum.
• Developed a methodology to investigating oligonucleotide interactions at subnanomolar level for antisense drug development.
• Label-free DNA array based on SPR imaging towards the parallelized screening of oligonucleotide interactions.
• Led a biosensor group (5 people, 2005-2007). Responsible for successful projects performance and results presentations. Managed laboratory technicians.

February 2002

Visiting Scientist, LPBC,Université Pierre et Marie Curie (Paris VI), Paris, France.

Fluorescence microscopy for study of interactions of oligonucleotides and cells.

Vzdělání:

2002 - 2006: Ph.D. : Charles University in Prague, Czech Republic, School of Mathematics and Physics, Division of Biomolecular Physics.
Dissertation: Monitoring of Biomolecular Interactions for Nucleic Acids Research.

1997 - 2002: M.S. : Charles University in Prague, Czech Republic, School of Mathematics and Physics, Division of Biomolecular Physics.
Thesis: Study of Thermodynamic Behavior of Oligonucleotide Complexes for Antisense Drugs Development By Means of Optical Spectroscopy

Ocenění:

2011: Czech L’ORÉAL-UNESCO Award for Women in Science

2008: Fellowship of the Division of Photonics of the IPE AS CR

2006: Bolzano’s Award of the Charles University in Prague for the best doctoral dissertation

2006: Europtrode VII, Tubingen, Germany – 3rd Poster Award

2005: Fellowship of the Division of Photonics of the IPE AS CR

2004: ASCOS - Advanced Study Course on Optical Chemical Sensors – Best Feasibility Award and Best Presentation Award

Patenty:

S. Jiang, Z. Zhang, S. Cheng, and H. Vaisocherová, Dual-functional Nonfouling Surfaces and Materials, WO/2008/083390.

Publikační činnost:

Knihy:

1. M. Piliarik, H. Vaisocherová and J. Homola, Surface Plasmon Resonance Biosensing, in Biosensors and Bioanalytical Technologies, A. Rasooly and J. Homola Editors, Humana Press, 2007, in print.
2. H. Vaisocherová and J. Homola, SPR Biosensors for Medical Diagnostics, J. Homola, Editor. Springer-Verlag: Berlin-Heidelberg-New York, 2006.
3. J. Štěpánek, H. Vaisocherová and M. Piliarik, Molecular Interactions in SPR Sensors, in Surface Plasmon Resonance Based Sensors, J. Homola, Editor. Springer-Verlag: Berlin-Heidelberg-New York, 2006.

Odborné články:

1. H. Vaisocherová, E. Brynda, J. Homola: Functionalizable Low-fouling coatings for label-free biosensing in complex biological media: advances and applications, Analytical and Bioanalytical Chemistry, 407, 3927-3953 (2015).
2. H. Vaisocherová, I. Víšová, M. Bocková, T. Špringer, M. L. Ermini, X. Song, Z. Krejčík, L. Chrastinová, O. Pastva, K. Pimková, M. Dostálová Merkerová, J. E. Dyr, and J. Homola: Rapid and sensitive detection of multiple MicroRNAs in cell lysate by low-fouling surface plasmon resonance biosensor, Biosensors and Bioelectronics, 70, 226–231 (2015).
3. H. Vaisocherová, A. D. Taylor, S. Jiang, K. Hegnerová, M. Vala, J. Homola, B. J. Yakes, J. Deeds, and S. DeGrasse, Journal of AOAC International, 94 (2011), 596 - 604.
4. T. Špringer, H. Šípová, H. Vaisocherová, J. Štěpánek, and J. Homola, Shielding Effect of Monovalent and Divalent Cations on Solid-Phase DNA Hybridization: Surface Plasmon Resonance Biosensor Study, Nucleic Acids Research, 38 (2010), 7343-51.
5. H. Šípová, H. Vaisocherová, J. Štěpánek, and J. Homola, A dual surface plasmon resonance assay for the determination of ribonuclease H activity, Biosensors & Bioelectronics, 26 (2010), 1605-11.
6. H. Vaisocherová, V. M. Faca, A. D. Taylor, S. Hanash, and S. Jiang, Comparative study of SPR and ELISA methods based on analysis of CD166/ALCAM levels in cancer and control human sera, Biosensors & Bioelectronics, 24 (2009), 2143 - 2148.
7. K. Hegnerová, M. Bocková, H. Vaisocherová, Z. Krištofiková, J.Říčný, D. Řípová, and J. Homola, Surface plasmon resonance biosensors for detection of Alzheimer disease biomarker, Sensors & Actuators B Chemical, 139 (2009), 69 - 73.
8. H. Vaisocherová, J. Snášel, T. Špringer, H. Šípová, I. Rosenberg, J. Štĕpánek, and J. Homola, Surface Plasmon Resonance Study on Monitoring of HIV-1 Integrase Activity, Analytical and Bioanalytical Chemistry, 393 (2009), 1165 - 1172.
9. H. Vaisocherová, W. Yang, Z. Zhang, Z. Cao, G. Cheng, S. Chen, A. D. Taylor, M. Piliarik, J. Homola, and S. Jiang, Ultra-low Fouling and Functionalizable Surface Chemistry Based on a Zwitterionic Polymer Enabling Sensitive and Specific Protein Detection in Undiluted Blood Plasma, Analytical Chemistry, 80 (2008),7894 - 7901 (published as Correspondence).
10. H. Vaisocherová, Z. Zhang, W. Yang, Z. Cao, G. Cheng, A. D. Taylor, M. Piliarik, J. Homola, and S. Jiang, Functionalizable Surface Platform with Reduced Nonspecific Protein Adsorption from Full Blood Plasma - Material Selection and Protein Immobilization Optimization, Biosensors & Bioelectronics, 24 (2008), 1924 - 1930.
11. W. Yang, S. Chen, G. Cheng, H. Vaisocherová, H. Xue, W. Li, J. Zhang, and S. Jiang, Film Thickness Dependence of Protein Adsorption from Blood Serum and Plasma onto Poly(sultobetaine)-grafted Surfaces, Lungmuir, 24 (2008), 9211-4.
12. Z. Zhang, H. Vaisocherová, W. Yang, H. Xue and S. Jiang, Nonfouling Behavior of Polycarboxybetaine-Grafted Surfaces: Environmental and Structural Effects, Biomacromolecules, 9 (2008), 2686-2692.
13. Z. Zhang, G. Cheng, L. Karr, H. Vaisocherová, and S. Jiang, Cationic Polycarboxybetaine Esters: Hydrolyzable to Zwitterionic Polycarboxybetaines with Controlled Biological Properties, Biomaterials, 29 (2008), 4719-25.
14. V. Ostatná, H. Vaisocherová, J. Homola, and T. Hianik, Effect of the immobilization of DNA aptamers on the detection of thrombin by means of surface plasmon resonance, Analytical and Bioanalytical Chemistry, 391 (2008), 1861-1869.
15. M. Piliarik, H. Vaisocherová, and J. Homola, Towards parallelized surface plasmon resonance sensor platform for sensitive detection of oligonucleotides, Sensors & Actuators B Chemical, 121 (2007), 187-193.
16. H. Vaisocherová, K. Mrkvová, M. Piliarik, P. Jinoch, M. Šteinbachová, and J. Homola, Surface plasmon resonance biosensor for direct detection of antibody against Epstein-Barr virus, Biosensors & Bioelectronics, 22 (2007), 1020-1026.
17. R. Slavík, J. Homola, and H. Vaisocherová, Advanced biosensing using simultaneous excitation of short and long range surface plasmons, Measurement Science and Technology, 17 (2006), 932 - 938.
18. H. Vaisocherová, A. Zítová, M. Lachmanová, J. Štěpánek, Š. Králíková, R. Liboska, D. Rejman, I. Rosenberg, and J. Homola, Investigating oligonucleotide interactions at subnanomolar level by surface plasmon resonance biosensor, Biopolymers, 82 (2006), 394 - 398.
19. M. Piliarik, H. Vaisocherová, and J. Homola: A new surface plasmon resonance sensor for high-throughput screening applications, Biosensors & Bioelectronics, 20 (2005), 2104 – 2110.
20. D. Němeček, H. Vaisocherová, J. Štěpánek, and P. – Y. Turpin, Structural features of a central mismatch in oligonucleotide hybrid duplexes visualized via Raman spectroscopy: model system for evaluation of potential "antisense" drugs, Biopolymers, 79 (2005), 1 - 8.
21. J. Dostálek, H. Vaisocherová, and J. Homola: Multichannel surface plasmon resonance biosensor with wavelength division multiplexing, Sensors & Actuators, 108 (2005), 758 - 764.
22. J. Homola, H. Vaisocherová, J. Dostálek, and M. Piliarik, Multianalyte surface plasmon resonance biosensing, Methods, 37 (2005), 26 - 36.

Příspěvky na konferencích:

1. H. Vaisocherová, Z. Zhang, G. Cheng, A. D. Taylor, W. Yang, M. Piliarik, J. Homola, S. Jiang, Super Low Fouling Zwitterionic Polymers for Sensor Applications, Europtrode IX, Book of Abstracts, Dublin, Ireland, 2008, 158.
2. S. Jiang, H. Vaisocherová, A. D. Taylor, W. Yang, B. J. Yakes, J. Deeds, S. Etheridge, K. Hegnerová, J. Homola. Sensitive surface plasmon resonance Biosensors for cancer biomarker discovery and food safety, oral presentation at the Europtrode IX, Book of Abstracts, Dublin, Ireland, 2008, 64.
3. K. Hegnerova, M. Bockova, H. Vaisocherová, Z. Kristofikova, J. Homola, Detection of biomarkers of Alzheimer’s disease by surface plasmon resonance, Europtrode IX, Book of Abstracts, Dublin, Ireland, 2008.
4. H. Šípová, H. Vaisocherová, J. Štěpánek, and J. Homola,: Surface plasmon resonance assay for quantitative determination of ribonuclease H activity. 20th Anniversary World Congress on Biosensors - Biosensors 2010, Glasgow, UK, May 26-28, 2010.
5. T. Springer, H. Šípová, H. Vaisocherová, J. Štěpánek and J. Homola, 2D Mapping of simultaneous Magnesium and Sodium Effect on DNA Hybridization via Surface Plasmon Resonance, In 12th European Conference on the Spectroscopy of Biological Molecules - Book of Abstracts, Bobigny, France, 2007.
6. M. Piliarik, H. Vaisocherová, and J. Homola, Surface plasmon imaging for parallelized monitoring of oligonucleotide hybridization, In 9th World Congress on Biosensors - Book of Abstracts, Toronto, Canada, 2006, 401.
7. P. Boltovets, H. Vaisocherová, M. Piliarik, and J. Homola, Detection of allergy markers by means of multichannel surface plasmon resonance biosensor, In 9th World Congress on Biosensors - Book of Abstracts, Toronto, Canada, 2006, 118.
8. H. Vaisocherová, A. Zítová, M. Lachmanová, P. Tobiška, and J. Homola, Monitoring oligonucleotide hybridization at subnanomolar level by surface plasmon resonance biosensor: Optimization of surface chemistry, In 9th World Congress on Biosensors - Book of Abstracts, Toronto, Canada, 2006, 400.
9. H. Vaisocherová, K. Mrkvová, M. Piliarik, M. Šteinbachová, P. Jinoch and J. Homola: Surface plasmon resonance biosensor for direct detection of antibody against Epstein-Barr virus, Europtrode VII, Book of Abstracts, Tubingen, Germany, 2006.
10. H. Vaisocherová, J. Štěpánek, I. Rosenberg and J. Homola: Investigating biomolecular interactions at subnanomolar level by surface plasmon resonance method, Oral Contribution In 11th European Conference on the Spectroscopy of Biological Molecules - Book of Abstracts, Aschaffenburg, Germany, 2005.
11. H. Vaisocherová, J. Štěpánek, I. Rosenberg and J. Homola: Investigation of oligonucleotide interactions by means of surface plasmon resonance technique, In 11th European Conference on the Spectroscopy of Biological Molecules - Book of Abstracts, Aschaffenburg, Germany, 2005, 162.
12. H. Vaisocherová, A. Zítová, M. Lachmanová, M. Piliarik, J. Štěpánek, and J. Homola: Investigating oligonucleotide interactions by SPR biosensor, Gordon Research Conference on Chemical Sensors and Interfacial Design, Oxford, United Kingdom 2005.
13. V. Ostatná, M. Sonlajtnerová, I. Grman, H. Vaisocherová, J. Homola, T. Hianik, Biosensor for thrombin based on DNA aptamers. A comparative analysis of the methods of detection and aptamer immobilization, In ESF Research Conference on Biological Surfaces and Interfaces, Sant Feliu de Guixols, Spain 2005.
14. H. Vaisocherová, J. Štěpánek, J. Bok and I. Rosenberg: Advanced data treatment improves spectroscopic results: UV absorption study of nucleic acids complexes, In 10th European Conference on the Spectroscopy of Biological Molecules - Book of Abstracts (Szalontai B., Kóta Z., Eds.), Hungarian Academy of Sciences, Szeged 2003, 151.