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LjuFhA

LIBRARY DESCRIPTION
LIBRARY CODE LjuFhA
LIBRARY TYPE BAC library
SPECIES, CULTIVAR Leptidea juvernica cv. not relevant
SPECIFICITY Female
DATE OF CONSTRUCTION 12/2014
PLACE OF ORIGIN Olomouc
AUTHORS Šafář
CLONING SITE HindIII
NUMBER OF CLONES 36 864
NUMBER OF PLATES 96
NUMBER OF LIGATIONS 3
AVERAGE INSERT SIZE 125kb
EMPTY CLONES 1.0%
GENOME/CHROMOSOME COVERAGE 6.6x
ANTIBIOTIC RESISTANCE Chloramphenicol (12.5 µg/ml)
HOST Escherichia coli MegaX DH10B T1
NOTES

B1: plates no.: 0001 - 0016, (6,144 clones, av. insert-size:107kb, coverage: 1.0x)
B2: plates no.: 0017 - 0085, (26,496 clones, av. insert-size:125kb, coverage: 4.7x)
M: plates no.: 0086 - 0096, (4,224 clones, av. insert-size:154kb, coverage: 0.9x)
BAC vector: pAGIBAC5 (R. Wing, AGI, ARIZONA)
Genome coverage is calculated based on estimated genome size of Leptidea spp. (700Mbp).

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* The resources are available for academic users by reimbursement of duplication costs. Academic and commercial users interested in copies of resouces should contact Dr. Jan Safar. Please note that we require signed MTA prior to shipment.
** SHIPMENT INFORMATION: Copy of BAC library is sent on dry ice via FedEx. Please, see a scheme for clone organization in 384-well plates.