Malínská K., Jelínková A., Petrášek J.
Plant Endosomes Methods and Protocols Methods in Molecular Biology
1209:
1-11,
2014
Klíčová slova:
FM dyes, endocytosis
Abstrakt:
FM (Fei-Mao) styryl dyes are compounds of amphiphilic character
that are used for the fluorescence tracking of endocytosis and related
processes, i.e., the internalization of membrane vesicles from the plasma
membrane (PM) and dynamics of endomembranes. Staining with FM
dyes and subsequent microscopical observations could be performed
both on the tissue and cellular level. Here, we describe simple procedures
for the effective FM dye staining and de-staining in root epidermal cells
of Arabidopsis thaliana seedlings and suspension-cultured tobacco cells.
The progression of FM dye uptake, reflected by an increased amount
of the dye in the endosomal compartments, is monitored under the
fluorescence microscope in a time-lapse manner. The data obtained can
be used for the characterization of the rate of endocytosis and the function
of components of endosomal recycling machinery.
Autoři z ÚEB: Adriana Jelínková,
Kateřina Malínská,
Jan Petrášek
Čeština
English