Akpinar, B.A., Lucas, S.J., Vrána, J., Doležel, J., Budak, H.
PLANT BIOTECHNOLOGY JOURNAL
13:
740-752,
2015
Klíčová slova:
D genome donor of wheat, chromosome 5D, comparative genomics
Abstrakt:
Flow cytometric sorting of individual chromosomes and chromosome-based sequencing reduces
the complexity of large, repetitive Triticeae genomes. We flow-sorted chromosome 5D of
Aegilops tauschii, the D genome donor of bread wheat and sequenced it by Roche 454 GS FLX
platform to approximately 2.2x coverage. Repetitive sequences represent 81.09% of the survey
sequences of this chromosome, and Class I retroelements are the prominent type, with a
particular abundance of LTR/Gypsy superfamily. Nonrepetitive sequences were assembled to
cover 17.76% of the total chromosome regions. Up to 6188 nonrepetitive gene loci were
predicted to be encoded by the 5D chromosome. The numbers and chromosomal distribution
patterns of tRNA genes suggest abundance in tRNALys and tRNAMet species, while the
nonrepetitive assembly reveals tRNAAla species as the most abundant type. A comparative
analysis of the genomic sequences of bread wheat and Aegilops chromosome 5D indicates
conservation of gene content. Orthologous unique genes, matching Aegilops 5D sequences,
numbered 3730 in barley, 5063 in Brachypodium, 4872 in sorghum and 4209 in rice. In this
study, we provide a chromosome-specific view into the structure and organization of the 5D
chromosome of Ae. tauschii, the D genome ancestor of bread wheat. This study contributes to
our understanding of the chromosome-level evolution of the wheat genome and presents a
valuable resource in wheat genomics due to the recent hybridization of Ae. tauschii genome
with its tetraploid ancestor.
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Autoři z ÚEB: Jaroslav Doležel,
Jan Vrána
Čeština
English