Čovanová M., Sauer M., Rychtář J., Friml J., Petrášek J., Zažímalová E.
PLoS ONE
8(7):
e70050,
2013
Klíčová slova:
Abstrakt:
Auxin binding protein 1 (ABP1) is a putative auxin receptor and its function is indispensable for plant
growth and development. ABP1 has been shown to be involved in auxin-dependent regulation of cell division and
expansion, in plasma-membrane-related processes such as changes in transmembrane potential, and in the
regulation of clathrin-dependent endocytosis. However, the ABP1-regulated downstream pathway remains elusive.
Using auxin transport assays and quantitative analysis of cellular morphology we
show that ABP1 regulates auxin efflux from tobacco BY-2 cells. The overexpression of ABP1can counterbalance
increased auxin efflux and auxin starvation phenotypes caused by the overexpression of PIN auxin efflux carrier.
Relevant mechanism involves the ABP1-controlled vesicle trafficking processes, including positive regulation of
endocytosis of PIN auxin efflux carriers, as indicated by fluorescence recovery after photobleaching (FRAP) and
pharmacological manipulations.
The findings indicate the involvement of ABP1 in control of rate of auxin transport across
plasma membrane emphasizing the role of ABP1 in regulation of PIN activity at the plasma membrane, and
highlighting the relevance of ABP1 for the formation of developmentally important, PIN-dependent auxin gradients.
Autoři z ÚEB: Milada Čovanová,
Jan Petrášek,
Eva Zažímalová
Čeština
English