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PLANT JOURNAL
84:
216-227,
2015
Keywords:
Barley, Hordeum vulgare L., BAC sequencing, gene distribution, recombination frequency, synteny, centromere BACs, HarvEST:Barley, Aegilops tauschii
Abstract:
Barley (Hordeum vulgare L.) possesses a large and highly repetitive genome of 5.1 Gb that has hindered the
development of a complete sequence. In 2012, the International Barley Sequencing Consortium released a
resource integrating whole-genome shotgun sequences with a physical and genetic framework. However,
because only 6278 bacterial artificial chromosome (BACs) in the physical map were sequenced, fine structure
was limited. To gain access to the gene-containing portion of the barley genome at high resolution, we identified
and sequenced 15 622 BACs representing the minimal tiling path of 72 052 physical-mapped gene-bearing
BACs. This generated ~1.7 Gb of genomic sequence containing an estimated 2/3 of all Morex barley
genes. Exploration of these sequenced BACs revealed that although distal ends of chromosomes contain
most of the gene-enriched BACs and are characterized by high recombination rates, there are also gene-dense
regions with suppressed recombination. We made use of published map-anchored sequence data from Aegilops
tauschii to develop a synteny viewer between barley and the ancestor of the wheat D-genome. Except
for some notable inversions, there is a high level of collinearity between the two species. The software HarvEST:
Barley provides facile access to BAC sequences and their annotations, along with the barley–
Ae. tauschii synteny viewer. These BAC sequences constitute a resource to improve the efficiency of marker
development, map-based cloning, and comparative genomics in barley and related crops. Additional knowledge
about regions of the barley genome that are gene-dense but low recombination is particularly relevant.
Fulltext: contact IEB authors
IEB authors: Jaroslav Doležel,
Hana Šimková
Čeština
English