Kobayashi, F., Wu, J., Kanamori, H., Tanaka, T., Katagiri, S., Karasawa, W., Kaneko, S., Watanabe, S., Sakaguchi, T., Hanawa, Y., Fujisawa, H., Kurita, K., Abe, C., Iehisa, J.C.M., Ohno, R., Šafář, J., Šimková, H., Mukai, Y., Hamada, M., Saito, M., Ishikawa, G., Katayose, Y., Endo, T.R., Takumi, S., Nakamura, T., Sato, K., Ogihara, Y., Hayakawa, K., Doležel, J., Nasuda, S., Matsumoto, T., Handa, H.
BMC GENOMICS
16:
595,
2015
Klíčová slova:
Centromere, Chromosomal rearrangement, Chromosome 6B, DNA marker, Gene order, Nucleolus organizer region, BAC physical map, RH map, Synteny, Wheat
Abstrakt:
Background: A complete genome sequence is an essential tool for the genetic improvement of wheat. Because
the wheat genome is large, highly repetitive and complex due to its allohexaploid nature, the International
Wheat Genome Sequencing Consortium (IWGSC) chose a strategy that involves constructing bacterial artificial
chromosome (BAC)-based physical maps of individual chromosomes and performing BAC-by-BAC sequencing.
Here, we report the construction of a physical map of chromosome 6B with the goal of revealing the structural
features of the third largest chromosome in wheat.
Results: We assembled 689 informative BAC contigs (hereafter reffered to as contigs) representing 91 % of the
entire physical length of wheat chromosome 6B. The contigs were integrated into a radiation hybrid (RH) map of
chromosome 6B, with one linkage group consisting of 448 loci with 653 markers. The order and direction of 480
contigs, corresponding to 87 % of the total length of 6B, were determined. We also characterized the contigs that
contained a part of the nucleolus organizer region or centromere based on their positions on the RH map and the
assembled BAC clone sequences. Analysis of the virtual gene order along 6B using the information collected for the
integrated map revealed the presence of several chromosomal rearrangements, indicating evolutionary events that
occurred on chromosome 6B.
Conclusions: We constructed a reliable physical map of chromosome 6B, enabling us to analyze its genomic
structure and evolutionary progression. More importantly, the physical map should provide a high-quality and
map-based reference sequence that will serve as a resource for wheat chromosome 6B.
Fulltext: kontaktujte autory z ÚEB
Autoři z ÚEB: Jaroslav Doležel,
Jan Šafář,
Hana Šimková
Čeština
English