Kobayashi, F., Katagiri, S., Karasawa, W., Hanawa, Y., Kanamori, H., Ito, Y., Fujisawa, H., Mukai, Y., Tanaka, T., Kaneko, S., Watanabe, S., Sakaguchi, T., Nasuda, S., Hayakawa, K., Abe, C., Ohno, R., Iehisa, J.C.M., Takumi, S., Doležel, J., Ogihara, Y., Matsumoto, T., Katayose, Y., Wu, J., Handa, H.
Advances in wheat genetics: From genome to field
:
101-107,
2015
Klíčová slova:
Abstrakt:
The International Wheat Genome Sequencing Consortium (IWGSC) has been
promoting the decoding of the wheat genome sequence to enhance our knowledge
of the structure and function of the wheat genome. The vision of the consortium is
to establish a high quality reference sequence with a strategy to sequence its whole
genome using BAC (bacterial artifi cial chromosome) clones aligned on the physical
map of each chromosome. Under the framework of IWGSC, we are in charge of the
chromosome 6B with the fi nancial support from the Japanese Ministry of Agriculture,
Forestry and Fisheries (“Genomics for Agricultural Innovation” project and
“Genomics-based Technology for Agricultural Improvement” project) and Nisshin
Flour Milling Inc. The “KomugiGSP” (Genome Sequence Program) ( http://komugigsp.
dna.affrc.go.jp/index.html ) currently consists of the following four main
research subjects: (1) Construction of 6B-specifi c BAC library and development of
DNA markers, (2) comprehensive analysis of transcripts for gene annotation, (3)
construction of the BAC physical map and genome sequencing (BAC-by-BAC and
whole chromosome survey sequencing), and (4) development of annotation pipeline.
Here, we report our current progresses on the construction of a physical map
for chromosome 6B using chromosome arms-specifi c BAC libraries with DNA
markers and BAC-based genomic sequencing.
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