Lucas, S.J., Šimková, H., Šafář, J., Jurman, I., Cattonaro, F., Vautrin, S., Bellec, A., Berges, H., Doležel, J., Budak, H.
FUNCTIONAL & INTEGRATIVE GENOMICS
12:
173–182,
2012
Klíčová slova:
Wheat, A genome, BAC end sequencing, Comparative genomics, Marker design
Abstrakt:
Bread wheat (Triticum aestivum L.) is one of the
most important crops globally and a high priority for genetic
improvement, but its large and complex genome has been
seen as intractable to whole genome sequencing. Isolation of
individual wheat chromosome arms has facilitated largescale
sequence analyses. However, so far there is no such
survey of sequences from the A genome of wheat. Greater
understanding of an A chromosome could facilitate wheat
improvement and future sequencing of the entire genome.
We have constructed BAC library from the long arm of T.
aestivum chromosome 1A (1AL) and obtained BAC end
sequences from 7,470 clones encompassing the arm. We
obtained 13,445 (89.99%) useful sequences with a cumulative
length of 7.57 Mb, representing 1.43% of 1AL and about
0.14% of the entire A genome. The GC content of the
sequences was 44.7%, and 90% of the chromosome was
estimated to comprise repeat sequences, while just over 1%
encoded expressed genes. From the sequence data, we
identified a large number of sites suitable for development of
molecular markers (362 SSR and 6,948 ISBP) which will
have utility for mapping this chromosome and for marker
assisted breeding. From 44 putative ISBP markers tested 23
(52.3%) were found to be useful. The BAC end sequence data
also enabled the identification of genes and syntenic blocks
specific to chromosome 1AL, suggesting regions of particular
functional interest and targets for future research.
Fulltext: kontaktujte autory z ÚEB
Autoři z ÚEB: Jaroslav Doležel,
Jan Šafář,
Hana Šimková
Čeština
English