Zatloukalová, P., Hřibová, E., Kubaláková, M., Suchánková, P., Šimková, H., Adoración, C., Kahl, G., Millán, T., Doležel, J.
CHROMOSOME RESEARCH
19:
729-739,
2011
Klíčová slova:
BAC-FISH, Chromosome isolation, Flow cytometric sorting, Fluorescence in situ hybridization, Genome mapping, Repetitive DNA
Abstrakt:
Cultivated chickpea is the third most
important legume after field bean and garden pea
worldwide. Despite considerable breeding towards
improved yield and resistance to biotic and abiotic
stresses, the production of chickpea remained stagnant,
but molecular tools are expected to increase the
impact of current improvement programs. As a first
step towards this goal, various genetic linkage maps
have been established and markers linked to resistance
genes been identified. However, until now, only
one linkage group (LG) has been assigned to a
specific chromosome. In the present work, mitotic
chromosomes were sorted using flow cytometry and
used as template for PCR with primers designed for
genomic regions flanking microsatellites. These primers
amplify sequence-tagged microsatellite site
markers. This approach confirmed the assignment of
LG8 to the smallest chromosome H. For the first time,
LG5 was linked to the largest chromosome A, LG4 to
a medium-sized chromosome E, while LG3 was
anchored to the second largest chromosome B.
Chromosomes C and D could not be flow-sorted
separately and were jointly associated to LG6 and
LG7. By the same token, chromosomes F and G were
anchored to LG1 and LG2. To establish a set of
preferably diagnostic cytogenetic markers, the
genomic distribution of various probes was verified
using FISH. Moreover, a partial genomic bacterial
artificial chromosome (BAC) library was constructed
and putative single/low-copy BAC clones weremapped cytogenetically. As a result, two clones were
identified localizing specifically to chromosomes E
and H, for which no cytogenetic markers were yet
available.
Fulltext: kontaktujte autory z ÚEB
Autoři z ÚEB: Jaroslav Doležel,
Eva Hřibová,
Marie Kubaláková,
Hana Šimková,
Pavla Suchánková
Čeština
English