Plant Reproduction Laboratory
Research Interests
We study a molecular background of flowering in the genus Chenopodium and cytoplasmic male sterility in Silene vulgaris. Both model plants are members of the same order –Caryophyllales. Interest in plant reproduction and plant evolution arose from previous projects focused on the genetic diversity of wild plant species.
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The genus Chenopodium contains both short-day and long-day plant species. A short-day species Chenopodium rubrum has been a traditional model of flowering investigated for a long time. A surprising feature of C. rubrum is its sensitivity to photoperiodic induction at seedling stage - at age of about five days. We have identified Chenopodium homologues of the genes CONSTANS and Flowering locus T, which play crucial roles in the course of photoperiodic induction of flowering in Arabidopsis thaliana. We study their expression in seedlings and mature plants of C. rubrum under various physiological conditions.
We noticed that FT- like genes could serve as molecular markers, particularly informative for phylogenetic studies of the complex group C. album sensu lato. -
The second project, currently starting in our lab, is an investigation of cytoplasmic male sterility in Silene vulgaris. We focus on variation in mitochondrial transcription among natural populations as well as on the basis of inheritance of mitochondrial DNA. We are searching for the genes encoding cytoplasmic male sterility in this species.
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We collaborate with Radomira Vankova who studies metabolism of cytokinins in transgenic tobacco under drought stress. We benefit from methods of plant gene expression analysis, adopted in the course of investigation of flowering (qRT PCR, Northerns with DIG labeled probes).
Workers
head of the laboratorysenior scientist
- RNDr. Helena Štorchová CSc.
graduated technical assistant
- Mgr. Lucie Černá
- Ing. Kateřina Haškovcová
postdoc
- Ing. Jana Drabešová PhD.
postgraduate student
- Ing. Pavla Koloušková
Research projects
- The role of hybridization and polyploidization in the evolution in Chenopodium album aggregate: From biosystematics to gene expression , GA ČR , Helena Štorchová
- Regulace kvetení u rodu Chenopodium studovaná pomocí transkriptomiky , GA ČR , Helena Štorchová
- Hunters or gardeners? Probing plant-microbe interactions in rootless carnivorous Utricularia from a transcriptomic perspective , GA ČR , Helena Štorchová
- Coexistence of native and inoculated arbuscular mycorrhizal fungi in the roots of host plants , GA ČR , Helena Štorchová
- Dynamic changes of mitochondrial DNA and transcription profiles in Silene vulgaris , GA ČR , Helena Štorchová
- The molecular genetic analysis of the candidate genes responsible for cytoplasmic male sterility in Silene vulgaris , MŠMT , Helena Štorchová
- The identification and studies of expression of the genes involved in photoperiodic flower induction in Chenopodium , GA ČR , Helena Štorchová
Publications
2010
- AQUATIC BOTANY 92 214-220 2010
- BMC PLANT BIOLOGY 10 11 2010 Fulltext: public PDF link
- FUNCTIONAL & INTEGRATIVE GENOMICS 10 547–559 2010
- 10 274 2010 Fulltext: public PDF link
2008
- PLANTA 228 929-940 2008 Fulltext: PDF link on intranet only
- PLANT, CELL AND ENVIRONMENT 31 341-353 2008 Fulltext: PDF link on intranet only
2007
- PLANT SYSTEMATICS AND EVOLUTION 268 235-256 2007 Fulltext: PDF link on intranet only
- AMERICAN FERN JOURNAL 97 149-154 2007 Fulltext: PDF link on intranet only
- BIOTECHNOLOGY AND SUSTAINABLE AGRICULTURE 2006 AND BEYOND 171-174 2007
2006
- BIOTECHNIQUES 41 156+ 2006 Fulltext: PDF link on intranet only