Laboratory of Stress Physiology
Field of research
The research of the laboratory is focused on plant responses to different environmental stresses (especially those connecting with climate global change: elevated CO2 concentration, drought, and UV-radiation, and on heat or chilling stress, and heavy metal) and to biotic stresses (ageing, high content of endogenous cytokinins, viral infection) acting individually or in different combinations.
The changes in physiological and structural characteristics are determined on the level of whole plant and leaf, cellular, or subcellular level. We determine parameters of gas exchange, water regime, composition and function of photosynthetic apparatus and other cell organelles, development of reactive oxygen species, oxidative damage and induction of biosynthesis of protecting pigments and antioxidants. Furthermore, the changes in metabolism of reactive nitrogen species during natural and stress induced senescence are investigated.
Not only immediate responses to environmental factors are followed but also acclimations and de-acclimations to stresses and after their relief.
Important direction of our research is to determine the effect of phytohormones abscisic acid and cytokinins (endogenous content and/or application) on plant responses to stresses.
Our lab offers certain methods in frame of cooperation or on a contract:
- detection of oxidative damage (to lipids and/or proteins),
- antioxidative defence enzymes (catalase, superoxide dismutase, ascorbate peroxidase, glutathione reductase)
- low molecuar antioxidants (ascorbate, glutathione)
- phenolic compounds, anthocyanins
- pigments (chlorophylls, carotenoids)
Workers
head of the laboratorysenior scientist
- RNDr Naďa Wilhelmová CSc
scientist
- RNDr. Helena Synková CSc.
scientific assistant
- Mgr. Daniel Haisel Ph.D.
postdoc
- Mgr. Renáta Schnablová Ph.D.
maternity leavepostgraduate student
- Mgr. Petra Spoustová
technician
former employee
- RNDr Tomáš Gichner DrSc
- Ing. Dagmar Procházková PhD.
Research projects
- Nitrosative stress during natural and zinc induced senescence , GA ČR , Dagmar Procházková
- Fotoinhibice a oxidativní stres v průběhu aklimatizace in vitro pěstovaných rostlin , GA ČR , Jana Pospíšilová
- Změny v projevu heteróze u kukuřice v důsledku sucha: proč kříženci reagují odlišně než rodiče? , GA ČR , Naďa Wilhelmová
Publications
2014
- JOURNAL OF PLANT PHYSIOLOGY 171 1625- 1633 2014
- Ahmad P. (ed.): Emerging Technologies and Management of Crop Stress Tolerance 249-266 2014
- VESMÍR 5 266-268 2014
- BIOLOGIA PLANTARUM 58 (4) 783-787 2014 Fulltext: PDF link on intranet only
2013
- PHOTOSYNTHETICA 51 483-489 2013
- JOURNAL OF EXPERIMENTAL BOTANY 64 2805-2815 2013 Fulltext: PDF link on intranet only
- BIOTECHNOLOGIA 94 192 2013
- CZECH JOURNAL OF GENETICS AND PLANT BREEDING 49 9-15 2013
- BIOLOGIA PLANTARUM 57 (4) 783-787 2013
- INTERNATIONAL JOURNAL of RADIATION RESEARCH 11 (4) 233-243 2013