Laboratory of Stress Physiology
Field of research
The research of the laboratory is focused on plant responses to different environmental stresses (especially those connecting with climate global change: elevated CO2 concentration, drought, and UV-radiation, and on heat or chilling stress, and heavy metal) and to biotic stresses (ageing, high content of endogenous cytokinins, viral infection) acting individually or in different combinations.
The changes in physiological and structural characteristics are determined on the level of whole plant and leaf, cellular, or subcellular level. We determine parameters of gas exchange, water regime, composition and function of photosynthetic apparatus and other cell organelles, development of reactive oxygen species, oxidative damage and induction of biosynthesis of protecting pigments and antioxidants. Furthermore, the changes in metabolism of reactive nitrogen species during natural and stress induced senescence are investigated.
Not only immediate responses to environmental factors are followed but also acclimations and de-acclimations to stresses and after their relief.
Important direction of our research is to determine the effect of phytohormones abscisic acid and cytokinins (endogenous content and/or application) on plant responses to stresses.
Our lab offers certain methods in frame of cooperation or on a contract:
- detection of oxidative damage (to lipids and/or proteins),
- antioxidative defence enzymes (catalase, superoxide dismutase, ascorbate peroxidase, glutathione reductase)
- low molecuar antioxidants (ascorbate, glutathione)
- phenolic compounds, anthocyanins
- pigments (chlorophylls, carotenoids)
Workers
head of the laboratorysenior scientist
- RNDr Naďa Wilhelmová CSc
scientist
- RNDr. Helena Synková CSc.
scientific assistant
- Mgr. Daniel Haisel Ph.D.
postdoc
- Mgr. Renáta Schnablová Ph.D.
maternity leavepostgraduate student
- Mgr. Petra Spoustová
technician
former employee
- RNDr Tomáš Gichner DrSc
- Ing. Dagmar Procházková PhD.
Research projects
- Nitrosative stress during natural and zinc induced senescence , GA ČR , Dagmar Procházková
- Fotoinhibice a oxidativní stres v průběhu aklimatizace in vitro pěstovaných rostlin , GA ČR , Jana Pospíšilová
- Změny v projevu heteróze u kukuřice v důsledku sucha: proč kříženci reagují odlišně než rodiče? , GA ČR , Naďa Wilhelmová
Publications
2011
- KOREAN J. ENVIRN. BIOL. 29 280-285 2011
- CHEMICKÉ LISTY XI. mezioborové setkání mladých biologů biochemiků a chemiků 105 407-408 2011
2010
- BIOLOGIA PLANTARUM 54 461-470 2010 Fulltext: PDF link on intranet only
- JOURNAL OF PLANT PHYSIOLOGY 167 1360-1370 2010
- HANDBOOK OF PLANT AND CROP STRESS, 3rd edition, Pessarakli M. (ed.), CRC Press Pp. 139-155 2010
2009
- BIOLOGIA PLANTARUM 53 11-20 2009
- PHOTOSYNTHETICA 47 409-414 2009
- BIOLOGIA PLANTARUM 53 691-696 2009
- BIOLOGIA PLANTARUM 53 475-483 2009
- BULLETIN ČESKÉ SPOLEČNOSTI EXPERIMENTÁLNÍ BIOLOGIE ROSTLIN 24-25 2009