Čeština
EnglishCyclin-dependent kinase inhibitor, roscovitine, in combination with exogenous cytokinin, N6-benzyladenine, causes increase of cis-cytokinins in immobilized tobacco cells
Blagoeva, Elitsa; Malbeck, Jiří; Gaudinová, Alena; Vaněk, Tomáš; Vaňková, Radomíra
BIOTECHNOLOGY LETTERS 25 [6]: 469-472, 2003
Keywords: alginate; cytokinins; plant cell immobilization
Abstract: Dynamics of the response of tobacco cells (line BY-2) to exogenous cytokinin, N6-benzyladenine, and cyclin-dependent kinase inhibitor, roscovitine, was followed using alginate-immobilized cells packed into a column. N6-Benzyladenine (1.25 μM) increased the synthesis of the physiologically-active endogenous cytokinin, isopentenyladenosine, in the effluent up to 0.1 nM. Simultaneously, conversion of the excess of endogenous cytokinins to biologically inactive derivatives of cis-zeatin occurred, up to 0.8 nM. Roscovitine (50 μM) further increased cis-cytokinins, up to 2.2 nM.
IEB authors: Alena Gaudinová, Jiří Malbeck, Tomáš Vaněk, Radomíra Vanková
BIOTECHNOLOGY LETTERS 25 [6]: 469-472, 2003
Keywords: alginate; cytokinins; plant cell immobilization
Abstract: Dynamics of the response of tobacco cells (line BY-2) to exogenous cytokinin, N6-benzyladenine, and cyclin-dependent kinase inhibitor, roscovitine, was followed using alginate-immobilized cells packed into a column. N6-Benzyladenine (1.25 μM) increased the synthesis of the physiologically-active endogenous cytokinin, isopentenyladenosine, in the effluent up to 0.1 nM. Simultaneously, conversion of the excess of endogenous cytokinins to biologically inactive derivatives of cis-zeatin occurred, up to 0.8 nM. Roscovitine (50 μM) further increased cis-cytokinins, up to 2.2 nM.