Intranet Čeština

Membrane Transport

Membrane Transport Sodium and potassium transporting protein observed in yeast cells through a fluorescence microscope. Green fluorescence corresponds to the protein localized in the cell plasma membrane; red fluorescence visualizes membranes of vacuoles.

We study the proteins transporting compounds and signals across the cell membranes. These proteins, called transporters, assure the uptake of nutrients into the cells, efflux of waste compounds from cells and communication with the environment.  To study the animal and plant transporters, we mainly use a model eukaryotic microorganism – yeasts. Our research is focused mainly on:

  • Structure, function and regulation of cell transport systems at protein molecule level.
  • Role of transporters in specific cell properties and in diseases.
  • Role of transporters in Candida virulence and pathogenicity.
  • Development of new techniques to estimate cell physiological parameters.

Projects

Transporters reflected in specific properties of nonconventional yeasts

Some yeast species survive extreme changes in the environmental pH, temperature or osmotic pressure. We aim to identify and characterize specific transporters whose activity contributes to the ability to survive adverse environmental conditions. Acquired knowledge will help to improve the properties of yeast species used in industrial processes. More

Transporters reflected in specific properties of nonconventional yeasts

Some yeast species survive extreme changes in the environmental pH, temperature or osmotic pressure. We aim to identify and characterize specific transporters whose activity contributes to the ability to survive adverse environmental conditions. Acquired knowledge will help to improve the properties of yeast species used in industrial processes. More

Cell cation and pH homeostasis

The intracellular concentration of potassium and sodium cations, as well as of protons, is strictly regulated via the activity of a series of membrane proteins that mediate the flux of cations and protons with various transport mechanisms. Incorrect functioning of some transporters results in serious disorders and diseases. We study in detail the roles of individual transporters and the impact of their activity on cell fitness. More

Achievements

The best poster award for Klara Richerova

Klara Richterova is the author of award-winning poster at 6th Czech-Swiss symposium BioTech 2014. The work with name „Heterologous expression of three laccases from different source of origin in yeast Saccharomyces cerevisiae and their use for environmental application.“ was born in collaboration with Institute of Organic Chemistry and Biochemistry AS CR. Congratulation!  More

Publications

Zimmermannová, Olga - Salazar, A. - Sychrová, Hana - Ramos, J. Zygosaccharomyces rouxii Trk1 is an efficient potassium transporter providing yeast cells with high lithium tolerance . FEMS Yeast Research. 2015, Vol. 15, 4, fov029 . IF = 2.818 [ASEP] [ doi ]
Rosas-Santiago, P. - Lagunas-Goméz, D. - Barkla, B. J. - Vera-Estrella, R. - Lalonde, S. - Jones, A. - Frommer, W. B. - Zimmermannová, Olga - Sychrová, Hana - Pantoja, O. Identification of rice cornichon as a possible cargo receptor for the Golgi-localized sodium transporter OsHKT1;3 . Journal of Experimental Botany. 2015, Vol. 66, 9, p. 2733-2748 . IF = 5.526 [ASEP] [ doi ]
Papoušková, Klára - Jiang, L. - Sychrová, Hana . Vcx1 and ESCRT components regulate intracellular pH homeostasis in the response of yeast cells to calcium stress . FEMS Yeast Research. 2015, Vol. 15, 2, fov007 . IF = 2.818 [ASEP] [ doi ]
Kodedová, Marie - Sychrová, Hana . Changes in the Sterol Composition of the Plasma Membrane Affect Membrane Potential, Salt Tolerance and the Activity of Multidrug Resistance Pumps in Saccharomyces cerevisiae . PLoS ONE. 2015, Vol. 10, 9, e0139306 . IF = 3.234 [ASEP] [ doi ]
Kinclová-Zimmermannová, Olga - Falson, P. - Cmunt, Denis - Sychrová, Hana . A Hydrophobic Filter Confers the Cation Selectivity of Zygosaccharomyces rouxii Plasma-Membrane Na (+)/H (+) Antiporter . Journal of Molecular Biology. 2015, Vol. 427, 8, p. 1681-1697 . IF = 4.333 [ASEP] [ doi ]

Article photogallery

Contacts

Fyziologický ústav AV ČR, v.v.i.
Department of Membrane Transport

Vídeňská 1083
14220 Praha 4
tel. +420 241 062 667
fax. +420 241 062 284
 

sychrova@fgu.cas.cz

People

RNDr. Hana Sychrová, DrSc.
head of the department
Prof. RNDr. Arnošt Kotyk, DrSc.
senior researcher
Ing. Olga Zimmermannová, Ph.D. (née Kinclová)
senior researcher
Ing. Zuzana Antošová, Ph.D. (née Chrastilová)
postdoc
  RNDr. Marie Kodedová, Ph.D.
postdoc
Mgr. Klára Papoušková, Ph.D. (née Velková)
postdoc

MSc. Vicent Liopis-Torregrosa
postdoc

Mgr. Hana Čuláková, PhD.
postdoc

Mgr. Michal Růžička, Ph.D.
lab manager
Mgr. Michala Dušková (née Bubnová)
PhD student
Mgr. Hana Elicharová
PhD student
Mgr. Barbora Hušeková
PhD student

Ing. Klára Herkommerová (née Richterová)

PhD student

Mgr. Petra Nevečeřalová

PhD student

Mgr. Kristýna Felcmanová

PhD student

Denis Cmunt
pregradual student
Pavla Herynková
lab technician

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