We investigate physiological regulations of metabolism and their disorders in obesity and its complications (type 2 diabetes, dislipidaemia...). In order to contribute to the treatment of these diseases, we test influence of pharmaceuticals, diet, and natural compounds, namely n-3 fatty acids of marine origin (omega-3). Our results demonstrate the importance of adipose tissue metabolism for fat accumulation and reveal new possibilities for prevention and treatment of obesity-related disorders by modulation metabolism of the tissue. As a general strategy, experimental findings from studies in mice are complemented by mechanistic studies in vitro, and they are further explored in clinical studies. Collaborations with industrial partners and small companies are essential for effective translational research. In cooperation with the producers of tested compounds in Czech Republic and Norway, we evaluate the potential of omega-3 for enhancement of the effects of selected pharmaceuticals and other compounds.