11.1. 2016, Membránový transport

Research topics: Yeasts are eukaryotic microoroganisms used in industry for the production of a wide range of products, but, due to the similarity of their cellular structure and processes to those of higher eukaryotes, they serve as an important model organism in modern cell biology research. Some yeast species are opportunistic human pathogens. Our research is focused on proteins transporting compounds and signals across the cell membranes (transporters) that assure the uptake of nutrients into the cells, efflux of waste compounds from cells and communication with the environment. Our current research includes:

• Molecular characterization of transporters from various yeast species as concern their structure/function, substrate specificity, transport mechanism, biogenesis/degradation and their role in cell physiology
• Yeast as a tool to study transport processes in animal and plant cells
• Transporters in pathogenic yeasts as potential antifungal drug targets
• Specific transporters in non-conventional yeast species and their application in biotechnology                                     

Candidate’s profile (requirements): M.Sc. or equivalent in molecular biology, microbiology or biochemistry, strong interest in research and experimental work, some relevant laboratory experience

PhD Project: Characterization of transporters and auxiliary proteins involved in cation and pH homeostases in various yeast cells. Transporters will be characterized in their origin cells (construction of knock-outs) and upon heterologous expression in Saccharomyces cerevisiae. Their substrate specificity, biogenesis and degradation will be elucidated with the aim to characterize all their roles in cell physiology. The experimental work will involve methods of molecular biology, biochemistry, bioinformatics and biophysics.

Supervisor:  Dr. Hana Sychrová

                      Dr. Olga Zimmermannová