Recording of electrophysiological activity from dorsal horn neurons in acute spinal cord slices with patch-clamp technique.
The main research interest of our department is to study mechanisms of pain and to explore new possibilities of pain treatment, especially in chronic states. Our experimental work is concentrated on the modulation of nociceptive information at the spinal cord level that is the relay center between the periphery and the higher brain areas. Our goal is to study these modulatory mechanisms in order to improve therapy for pain conditions, such as allodynia, hyperalgesia, neuropathic and cancer related pain. Our focus is on the role of TRPV1 receptors and inflammatory cytokines. In our research we use mainly electrophysiological, immunohistochemical and behavioral methods. Another project is directed towards the role of thyroid hormones (THs) and omega-3-polyunsaturated fatty acid (n-3 PUFA) in the physiology of cardiac and skeletal muscles. Models of hyper- and hypothyroid status are used to analyze potential influence of n-3 PUFA on the induced pathological changes.
Projects
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We study the effect of n-3 PUFA on physiological parameter changes induced by altered thyroid hormone levels.
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N-acylphosphatidylethanolamine (NAPE), as the precursor of anandamide, an endogenous agonist of TRPV1 and cannabinoid receptors, could play a role in modulation of nociceptive signalization in the spinal cord.
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Achievements
Altogether 70 scientists from 17 foreign countries and 6 from the Czech Republic have attended the meeting as registered participants.
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Publications
Soukup, Tomáš - Smerdu, V.
Effect of altered innervation and thyroid hormones on myosin heavy chain expression and fiber type transitions: a mini-review
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Histochemistry and Cell Biology. 2015, Vol. 143, 2, p. 123-130
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IF = 2.927
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Soukup, Tomáš - Diallo, Michael
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Proportions of myosin heavy chain mRNAs, protein isoforms and fiber types in the slow and fast skeletal muscles are maintained after alterations of thyroid status in rats
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Physiological Research. 2015, Vol. 64, 1, p. 111-118
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IF = 1.293
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Li, Y. - Adámek, Pavel - Zhang, H. - Tatsui, C. E. - Rhines, L. D. - Mrózková, Petra - Li, Q. - Kosturakis, A. K. - Cassidy, R. M. - Harrison, D. S. - Cata, J. P. - Sapire, K. - Zhang, H. - Kennamer-Chapman, R. M. - Jawad, A. B. - Ghetti, A. - Yan, J. - Paleček, Jiří - Dougherty, P. M.
The Cancer Chemotherapeutic Paclitaxel Increases Human and Rodent Sensory Neuron Responses to TRPV1 by Activation of TLR4
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Journal of Neuroscience. 2015, Vol. 35, 39, p. 13487-13500
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IF = 6.344
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Uchytilová, Eva - Špicarová, Diana - Paleček, Jiří
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TRPV1 antagonist attenuates postoperative hypersensitivity by central and peripheral mechanisms
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Molecular Pain 2014, roč. 10, 2014, p. 67. ISSN 1744-8069.
IF = 3.654
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doi
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Špicarová, Diana - Nerandžič, Vladimír - Paleček, Jiří
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Update on the role of spinal cord TRPV1 receptors in pain modulation
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Physiological Research 2014, roč. 63, suppl.1, s225-s236. ISSN 0862-8408.
IF = 1.293
[ASEP]
Špicarová, Diana - Adámek, Pavel - Kalynovska, Nataliia - Mrózková, Petra - Paleček, Jiří
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TRPV1 receptor inhibition decreases CCL2-induced hyperalgesia
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Neuropharmacology 2014, roč. 81, jun, p. 75-84. ISSN 0028-3908.
IF = 5.106
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Soukup, Tomáš
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Effects of long-term thyroid hormone level alterations, n-3 polyunsaturated fatty acid supplementation and statin administration in rats
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Physiological Research 2014, roč. 63, suppl.1, s119-s131. ISSN 0862-8408.
IF = 1.293
[ASEP]
Kopecká, Kateřina - Zachařová, Gisela - Smerdu, V. - Soukup, Tomáš
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Slow to fast muscle transformation following heterochronous isotransplantation is influenced by host thyroid hormone status
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Histochemistry and Cell Biology 2014, roč. 142, 6, p. 677-684. ISSN 0948-6143.
IF = 3.054
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Žurmanová, J. - Soukup, Tomáš
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Comparison of myosin heavy chain mRNAs, protein isoforms and fiber type proportions in the rat slow and fast muscles
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Physiological Research, 2013, Vol. 61, 4, p. 445-453
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IF = 1.531
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Rauchová, Hana - Vokurková, Martina - Pavelka, Stanislav - Behuliak, Michal - Tribulová, N. - Soukup, Tomáš
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N-3 Polyunsaturated Fatty Acids Supplementation Does not Affect Changes of Lipid Metabolism Induced in Rats by Altered Thyroid Status
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Hormone and Metabolic Research, 2013, Vol. 45, 7, p. 507-512
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IF = 2.145
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An important approach in our research is recording of electrophysiological activity from dorsal horn neurons in acute spinal cord slices with patch-clamp technique. This method allows us recording of synaptic activity in the dorsal horn, where the nociceptive information between the peripheral and central nervous system is transmitted. The left image shows dorsal horn in spinal cord slice with a patch microelectrode at low magnification; on the right one a recorded neuron is visible under higher magnification.
For the study of cardiac and skeletal muscles histochemical and immunocytochemical staining of tissue sections is used. On the picture skeletal muscle sections stained for specific muscle proteins is shown enabling us quantitative analysis of different muscle fiber types (převzato z práce Soukup et al., Physiol Res 61: 575-586, 2012).
