Laboratory is focused on the research of membrane receptors and secretion in neuroendocrine cells, with a special emphasis on the interactions between plasma membrane electrical events and receptor-controlled pathways. The Laboratory investigates signaling pathways at the cellular and molecular levels, determines the manner in which hormones and neurotransmitters utilize calcium and cyclic nucleotides as intracellular messengers, and characterizes channels involved in electrical activity, calcium signaling, and release of hormones and neurotransmitters.
Main topics:
- Ion channels and electrical excitability of pituitary cells
- Purinergic signaling in neurons of suprachiasmatic and supraoptic nuclei of hypothalamus
- Relationship between structure and function of purinergic P2X receptor-channels
Projects
Conflicting reports exist regarding ATP modulation of synaptic transmission and electrical activity in vasopressin- and oxytocin-containing neurons from supraoptic nucleus (SON). We are using electrophysiological methods and calcium imaging on hypothalamic neurons of rat brain slices to study these properties.
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Purinergic P2X receptors (P2X1-7) are ligand-gated ion channels which binding sites for allosteric modulators are still not well understood. Activation of these receptors is the major signaling pathway of extracellular adenosine 5'-triphosphate (ATP) in neuroendocrine cells.
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Achievements
Publications
Zemková, Hana - Khadra, A. - Rokic, Milos Boro - Tvrdoňová, Vendula - Sherman, A. - Stojilkovic, S. S.
Allosteric regulation of the P2X4 receptor channel pore dilation
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Pflugers Archiv - European Journal of Physiology. 2015, Vol. 467, 4, p. 713-726
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IF = 4.101
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doi
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Jindřichová, Marie - Bhattacharya, Anirban - Rupert, Marian - Skopek, Petr - Obšil, T. - Zemková, Hana
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Functional characterization of mutants in the transmembrane domains of the rat P2X7 receptor that regulate pore conductivity and agonist sensitivity
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Journal of Neurochemistry. 2015, Vol. 133, 6, p. 815-827
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IF = 4.281
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doi
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Zemková, Hana - Tvrdoňová, Vendula - Bhattacharya, Anirban - Jindřichová, Marie
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Allosteric modulation of ligand gated ion channels by ivermectin
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Physiological Research 2014, roč. 63, suppl.1, s215-s224. ISSN 0862-8408.
IF = 1.293
[ASEP]
Tvrdoňová, Vendula - Rokic, Milos Boro - Stojilkovic, S. S. - Zemková, Hana
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Identification of Functionally Important Residues of the Rat P2X4 Receptor by Alanine Scanning Mutagenesis of the Dorsal Fin and Left Flipper Domains
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PLoS ONE 2014, roč. 9, 11, e112902. ISSN 1932-6203.
IF = 3.234
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doi
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Rokic, Milos Boro - Stojilkovic, S. S. - Zemková, Hana
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Structural and functional properties of the rat P2X4 purinoreceptor extracellular vestibule during gating
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Frontiers in Cellular Neuroscience 2014, roč. 8, jan 29, p. 3. ISSN 1662-5102.
IF = 4.289
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doi
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Cellular localization of P2X2 receptors in supraoptic nuclei of hypothalamus Immunohistochemistry on the rat hypothalamic slices containing supraoptic nucleus and optic chiasm (OC). P2X2R immunoreactivity (green) is observed in somata of the supraoptic neurons. Staining was performed using antibodies against the P2X2R (green), neuronal marker NeuN (red) and glia cell marker GFAP (blue). Scale is 50 µm.
