Proteins of tooth pulp separated by 2D electrophoresis (excision) and stained with silver.
Main topics:
- Instrumentation progress in the area of separation methods
- Methodological progress and development of new separation methods applicable to the estimation of physiologically important compounds (capillary electrophoresis and HPLC/MS).
- Posttranslational changing of structural proteins (mainly connective tissue)
- Developing of separation methods for study of proteins and peptides
Capillary electrophoresis: two systems by Beckman (5500 and 5000) with three detectors: UV, DAD and LIF (laser-induced fluorescence)
CE-MS coupling (Beckman P/ACE 5000 and Agilent MSD 1100)
