In this paper, new steps toward a etter understanding and utilization of high-pressure high-temperature nanodiamonds (NDs) containing nitrogen-vacancy (NV) centers have been taken. NV–-related long-term luminescence of oxygenated particles increased in comparison to plasma hydrogenated NDs’ NV– luminescence. The optically detected NV– electron spin resonance process can be also significantly affected by ND termination. For H-terminated ND particles the NV– to NV0 conversion energy is lower than the NV– excitation energy, so that the delocalized triplet electrons can be more easily released from the original positions and drawn to the electron-attracting localities in the material. The final result of this study was application of luminescent NDs in cells, showing the detectability of luminescent NDs in a standard confocal microscope and ND subcellular distribution in the cells by TEM.
Nanodiamonds in cell – transmission electron microscopy image. Nanodiamonds are localized in cytoplasm (C) outside the nucleus (N).
1 Institute of Physics, Academy of Sciences Czech Republic v.v.i, Na Slovance 2, CZ-182 21 Prague 8, Czech Republic
2 Faculty of Mathematics and Physics, Charles University in Prague, Ke Karlovu 3, CZ-121 16 Prague 2, Czech Republic
3 Faculty of Nuclear Science and Physical Engineering, Czech Technical University in Prague, Brehova 7, CZ-115 19 Prague 1, Czech Republic
4 Institute of Organic Chemistry and Biochemistry, Academy of Sciences Czech Republic, v.v.i., Flemingovo náměstí 2, CZ-166 10 Prague 6, Czech Republic
5 Faculty of Chemistry, Materials Research Centre, Brno University of Technology, Purkyňova 118, CZ-612 00 Brno, Czech Republic
6 Institute of Physics, Kazan Federal University, Kremlevskaya 18, 420008 Kazan, Russia
7 J. Heyrovsky Institute of Physical Chemistry, Academy of Sciences Czech Republic v.v.i, Dolejškova 2155/3, CZ-182 23 Prague 8, Czech Republic
8 Institute of Microbiology, Academy of Sciences Czech Republic, v.v.i., Vídeňská 1083, CZ-142 20 Prague 4, Czech Republic
9 Institute of Pathological Physiology, First Faculty of Medicine, Charles University in Prague, Kateřinská 32, CZ-121 08 Prague 2, Czech Republic
10 Department of Pharmacology and Immunotherapy, Veterinary Research Institute, CZ-621 00 Brno, Czech Republic
11 Faculty of Biomedical Engineering, Czech Technical University in Prague, Nam. Sı́tná 3105, 272 01 Kladno, Czech Republic
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