Intranet Čeština

Protein Structures

Protein Structures

http://www.biomed.cas.cz/d312/index.php?section=home

Our group is focused on structural biology (the relationship between the structure and function of certain groups of proteins), particularly we focus on the proteins which participate in the signal transmission in the cell. Among methods we use are recombinant protein expression, biophysical characterization, study of intermolecular interactions, protein structure and interaction surfaces. All these methods enable us to better understand the details how the activity and function of protein-protein complexes is regulated. Our research is focused mainly on:

 

  • Structural biology of 14-3-3 proteins and their complexes
  • Study of DNA-binding domain of transcription factor FOXO4
  • Mechanism of regulation of Ask kinases
  • Interaction  of cytoplasmatic domains of TRP channels

Projects

Calmodulin and S100A1 protein interact with N-terminus of TRPM3 channel

Transient receptor potential (TRP) channels are a wide family of non-selective ion channels responsible for monovalent and divalent cation influx into the cells. Members of this family are involved in many sensory processes. We identified several positively charged residues as having a crucial impact on CaM/S100A1 binding.  More

Achievements

Tomáš Obšil was awarded the title of professor

Prof. RNDr. Tomáš Obšil, Ph.D. was awarded by the title of professor at The Faculty of Science, Charles University, majoring in Physical Chemistry. Professors graduation will take place December 18, 2014. More

Publications

Kacířová, Miroslava - Košek, Dalibor - Kádek, Alan - Man, Petr - Večeř, J. - Herman, P. - Obšilová, Veronika - Obšil, Tomáš . Structural Characterization of Phosducin and Its Complex with the 14-3-3 Protein . Journal of Biological Chemistry. 2015, Vol. 290, 26, p. 16246-16260 . IF = 4.573 [ASEP] [ doi ]
Gryčová, Lenka - Holendová, Blanka - Lánský, Zdeněk - Bumba, Ladislav - Jirků, Michaela - Boušová, Kristýna - Teisinger, Jan . Ca2+ Binding Protein S100A1 Competes with Calmodulin and PIP2 for Binding Site on the C-Terminus of the TPRV1 Receptor . ACS Chemical Neuroscience. 2015, Vol. 6, 3, p. 386-392 . IF = 4.362 [ASEP] [ doi ]
Boušová, Kristýna - Jirků, Michaela - Bumba, Ladislav - Bednárová, Lucie - Šulc, Miroslav - Franěk, M. - Vyklický ml., Ladislav - Vondrášek, Jiří - Teisinger, Jan . PIP2 and PIP3 interact with N-terminus region of TRPM4 channel . Biophysical Chemistry. 2015, Vol. 205, Oct 2015, p. 24-32 . IF = 1.986 [ASEP] [ doi ]
Obšilová, Veronika - Kopecká, Miroslava - Košek, Dalibor - Kacířová, M. - Kylarová, Salome - Řežábková, L. - Obšil, T. Mechanisms of the 14-3-3 protein function: regulation of protein function through conformational modulation . Physiological Research 2014, roč. 63, suppl.1, s155-s164. ISSN 0862-8408. IF = 1.293 [ASEP]
Košek, Dalibor - Kylarová, Salome - Pšenáková, Katarína - Řežábková, L. - Herman, P. - Večeř, J. - Obšilová, Veronika - Obšil, T. Biophysical and Structural Characterization of the Thioredoxin-binding Domain of Protein Kinase ASK1 and Its Interaction with Reduced Thioredoxin . Journal of Biological Chemistry 2014, roč. 289, 35, p. 24463-24474. ISSN 0021-9258. IF = 4.573 [ASEP] [ doi ]
Kopecká, Miroslava - Košek, Dalibor - Kukačka, Zdeněk - Řežábková, Lenka - Man, Petr - Novák, Petr - Obšil, T. - Obšilová, Veronika . Role of the EF-hand-like Motif in the 14-3-3 Protein- mediated Activation of Yeast Neutral Trehalase Nth1 . Journal of Biological Chemistry 2014, roč. 289, 20, p. 13948-13961. ISSN 0021-9258. IF = 4.573 [ASEP] [ doi ]
Wald, Tomáš - Osičková, Adriana - Šulc, Miroslav - Benada, Oldřich - Semerádtová, A. - Řežábková, Lenka - Veverka, Václav - Bednárová, Lucie - Malý, J. - Macek, Pavel - Šebo, Peter - Slabý, Ivan - Vondrášek, Jiří - Osička, Radim . Intrinsically Disordered Enamel Matrix Protein Ameloblastin Forms Ribbon-like Supramolecular Structures via an N-terminal Segment Encoded by Exon 5 . Journal of Biological Chemistry, 2013, Vol. 288, 31, p. 22333-22345 . IF = 4.651 [ASEP] [ doi ]
Vácha, P. - Zusková, Iva - Bumba, Ladislav - Večeř, J. - Obšilová, Veronika - Obšil, T. Detailed kinetic analysis of the interaction between the FOXO4–DNA-binding domain and DNA . Biophysical Chemistry, 2013, Vol. 184, DEC 31, p. 68-78 . IF = 2.283 [ASEP] [ doi ]
Macáková, Eva - Kopecká, Miroslava - Kukačka, Zdeněk - Veisová, Dana - Novák, Petr - Man, Petr - Obšil, T. - Obšilová, Veronika . Structural basis of the 14-3-3 protein-dependent activation of yeast neutral trehalase Nth1 . Biochimica et Biophysica Acta. General Subjects, 2013, Vol. 1830, 10, p. 4491-4499 . IF = 3.848 [ASEP] [ doi ]
Janoušková, Hana - Ray, A.M. - Noulet, F. - Lelong-Rebel, I. - Choulier, L. - Schaffner, F. - Lehmann, M. - Martin, S. - Teisinger, Jan - Dontenwill, M. Activation of p53 pathway by Nutlin-3a inhibits the expression of the therapeutic target alpha 5 integrin in colon cancer cells . Cancer letters, 2013, Vol. 336, 2, p. 307-318 . IF = 4.258 [ASEP] [ doi ]

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Contacts

Institute of Physiology AS CR, v.v.i.
Department of Protein Structures

Vídeňská 1083
14220 Prague 4
tel. +420 241 062 191
fax. +420 241 062 477
 

veronika.obsilova@fgu.cas.cz

People

RNDr. Veronika Obšilová, Ph.D.
head of the department

Prof. RNDr. Tomáš Obšil, Ph.D.
senior researcher

RNDr. Miroslava Alblová (born Kopecká), Ph.D.
junior researcher
Ing. Jan Teisinger, CSc.
senior researcher
Ing. Michaela Jirků
PhD student
Mgr. Kristýna Boušová
PhD student
Mgr. Salome Kylarová
PhD student
Mgr. Miroslava Kacířová
PhD student
Mgr. Dana Kalábová (born Žáková) 
PhD student

Mgr. Olívia Petrvalská
PhD student

Mgr. Katarína Pšenáková
PhD student
Bc. Vojtěch Dolejš
undergraduate student
Bc. Jiří Šimůnek
undergraduate student
Bc. Aneta Šmídová
undergraduate student
Bc. Kateřina Jarosilová
undergraduate student
Simona Krausová
lab technician

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