Kralova, T., Cegan, R., Kubat, Z., Vrána, J., Vyskot, B., Vogel, I., Kejnovsky, E., Hobza, R.
CYTOGENETIC AND GENOME RESEARCH
143:
87-95,
2014
Keywords:
Microdissection, Sex chromosomes, Silene latifolia (white campion), Retrotransposons
Abstract:
Silene latifolia is a dioecious plant species with chromosomal
sex determination. Although the evolution of sex chromosomes
in S. latifolia has been the subject of numerous
studies, a global view of X chromosome structure in this
species is still missing. Here, we combine X chromosome
microdissection and BAC library screening to isolate new X
chromosome-linked sequences. Out of 8 identified BAC
clones, only BAC 86M14 showed an X-preferential signal after
FISH experiments. Further analysis revealed the existence
of the Athila retroelement which is enriched in the X
chromosome and nearly absent in the Y chromosome.
Based on previous data, the Athila retroelement belongs to
the CL3 group of most repetitive sequences in the S. latifolia
genome. Structural, transcriptomics and phylogenetic analyses
revealed that Athila CL3 represents an old clade in the
Athila lineage. We propose a mechanism responsible for
Athila CL3 distribution in the S. latifolia genome.
Fulltext: contact IEB authors
IEB authors: Roman Hobza,
Jan Vrána
