Čeština
EnglishGenomic damage induced in tobacco plants by chlorobenzoic acids - Metabolic products of polychlorinated biphenyls
Gichner T., Lovecká P., Vrchotová B.
MUTATION RESEARCH - GENETIC TOXICOLOGY AND ENVIRONMENTAL MUTAGENESIS 657: 140-145, 2008
Klíčová slova: Comet assay, Delor 103, Ethyl methanesulphonate, Nicotiana tabacum L. var. xanthi, Somatic mutations
Abstrakt: Tobacco seedlings (Nicotiana tabacum var. xanthi)were treated for 24 h with mono-(2- and 3-CBA), di-(2,5- and 3,4-CBA), and tri-(2,4,6- and 2,3,5-CBA)-chlorobenzoic acids (CBAs) and with the mixture of polychlorinated biphenyls – Delor 103, or cultivated for 1 or 2 weeks in soil polluted with the CBAs. DNA damage in nuclei of leaves and roots was evaluated by the comet assay. A significant increase in DNA damage was observed only at concentrations of CBAs that caused withering of leaves or had lethal effects within 2–4 weeks after the treatments. As the application of CBAs did not induce somatic mutations, the induced DNA migration is probably caused by necrotic DNA fragmentation and not by DNA damage resulting in genetic alteration. In contrast, the application of the monofunctional alkylating agent ethyl methanesulphonate as a positive control resulted in a dose–response increase of DNA damage and an increase of somatic mutations. Thus, the EMS-produced DNA migration is probably associated with genotoxin-induced DNA fragmentation. The data demonstrate that the comet assay in plants should be conducted together with toxicity studies to distinguish between necrotic and genotoxin-induced DNA fragmentation. The content of 2,5-CBA in tobacco seedlings was measured by reverse-phase high pressure liquid chromatography.
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Autoři z ÚEB: Tomáš Gichner
MUTATION RESEARCH - GENETIC TOXICOLOGY AND ENVIRONMENTAL MUTAGENESIS 657: 140-145, 2008
Klíčová slova: Comet assay, Delor 103, Ethyl methanesulphonate, Nicotiana tabacum L. var. xanthi, Somatic mutations
Abstrakt: Tobacco seedlings (Nicotiana tabacum var. xanthi)were treated for 24 h with mono-(2- and 3-CBA), di-(2,5- and 3,4-CBA), and tri-(2,4,6- and 2,3,5-CBA)-chlorobenzoic acids (CBAs) and with the mixture of polychlorinated biphenyls – Delor 103, or cultivated for 1 or 2 weeks in soil polluted with the CBAs. DNA damage in nuclei of leaves and roots was evaluated by the comet assay. A significant increase in DNA damage was observed only at concentrations of CBAs that caused withering of leaves or had lethal effects within 2–4 weeks after the treatments. As the application of CBAs did not induce somatic mutations, the induced DNA migration is probably caused by necrotic DNA fragmentation and not by DNA damage resulting in genetic alteration. In contrast, the application of the monofunctional alkylating agent ethyl methanesulphonate as a positive control resulted in a dose–response increase of DNA damage and an increase of somatic mutations. Thus, the EMS-produced DNA migration is probably associated with genotoxin-induced DNA fragmentation. The data demonstrate that the comet assay in plants should be conducted together with toxicity studies to distinguish between necrotic and genotoxin-induced DNA fragmentation. The content of 2,5-CBA in tobacco seedlings was measured by reverse-phase high pressure liquid chromatography.
Fulltext: kontaktujte autory z ÚEB
Autoři z ÚEB: Tomáš Gichner