Laboratory is focused on the research of membrane receptors and secretion in neuroendocrine cells, with a special emphasis on the interactions between plasma membrane electrical events and receptor-controlled pathways. The Laboratory investigates signaling pathways at the cellular and molecular levels, determines the manner in which hormones and neurotransmitters utilize calcium and cyclic nucleotides as intracellular messengers, and characterizes channels involved in electrical activity, calcium signaling, and release of hormones and neurotransmitters.
Main topics:
- Ion channels and electrical excitability of pituitary cells
- Purinergic signaling in neurons of suprachiasmatic and supraoptic nuclei of hypothalamus
- Relationship between structure and function of purinergic P2X receptor-channels
Projects
Conflicting reports exist regarding ATP modulation of synaptic transmission and electrical activity in vasopressin- and oxytocin-containing neurons from supraoptic nucleus (SON). We are using electrophysiological methods and calcium imaging on hypothalamic neurons of rat brain slices to study these properties.
More
Purinergic P2X receptors (P2X1-7) are ligand-gated ion channels which binding sites for allosteric modulators are still not well understood. Activation of these receptors is the major signaling pathway of extracellular adenosine 5'-triphosphate (ATP) in neuroendocrine cells.
More
Achievements
Publications
Zemková, Hana - Khadra, A. - Rokic, Milos Boro - Tvrdoňová, Vendula - Sherman, A. - Stojilkovic, S. S.
Allosteric regulation of the P2X4 receptor channel pore dilation
.
Pflugers Archiv - European Journal of Physiology. 2015, Vol. 467, 4, p. 713-726
.
IF = 4.101
[ASEP]
[
doi
]
Jindřichová, Marie - Bhattacharya, Anirban - Rupert, Marian - Skopek, Petr - Obšil, T. - Zemková, Hana
.
Functional characterization of mutants in the transmembrane domains of the rat P2X7 receptor that regulate pore conductivity and agonist sensitivity
.
Journal of Neurochemistry. 2015, Vol. 133, 6, p. 815-827
.
IF = 4.281
[ASEP]
[
doi
]
Zemková, Hana - Tvrdoňová, Vendula - Bhattacharya, Anirban - Jindřichová, Marie
.
Allosteric modulation of ligand gated ion channels by ivermectin
.
Physiological Research 2014, roč. 63, suppl.1, s215-s224. ISSN 0862-8408.
IF = 1.293
[ASEP]
Tvrdoňová, Vendula - Rokic, Milos Boro - Stojilkovic, S. S. - Zemková, Hana
.
Identification of Functionally Important Residues of the Rat P2X4 Receptor by Alanine Scanning Mutagenesis of the Dorsal Fin and Left Flipper Domains
.
PLoS ONE 2014, roč. 9, 11, e112902. ISSN 1932-6203.
IF = 3.234
[ASEP]
[
doi
]
Rokic, Milos Boro - Stojilkovic, S. S. - Zemková, Hana
.
Structural and functional properties of the rat P2X4 purinoreceptor extracellular vestibule during gating
.
Frontiers in Cellular Neuroscience 2014, roč. 8, jan 29, p. 3. ISSN 1662-5102.
IF = 4.289
[ASEP]
[
doi
]
Bendová, Zdeňka - Janoušková, Hana - Svobodová, Irena
.
Circadian rhythm and photic induction of the C-terminal splice variant of NMDAR1 subunit in the rat suprachiasmatic nucleus
.
Synapse 2014, roč. 68, 2, p. 85-88. ISSN 0887-4476.
IF = 2.127
[ASEP]
[
doi
]
Zemková, Hana - Kučka, M. - Bjelobaba, I. - Tomic, M. - Stojilkovic, S. S.
Multiple Cholinergic Signaling Pathways in Pituitary Gonadotrophs
.
Endocrinology, 2013, Vol. 154, 1, p. 421-433
.
IF = 4.717
[ASEP]
[
doi
]
Rokic, Milos Boro - Stojilkovic, S. S. - Vávra, Vojtěch - Kuzyk, Pavlo - Tvrdoňová, Vendula - Zemková, Hana
.
Multiple Roles of the Extracellular Vestibule Amino Acid Residues in the Function of the Rat P2X4 Receptor
.
PLoS ONE, 2013, Vol. 8, 3, e59411
.
IF = 3.730
[ASEP]
[
doi
]
Khadra, A. - Tomic, M. - Yan, Z. - Zemková, Hana - Sherman, A. - Stojilkovic, S. S.
Dual Gating Mechanism and Function of P2X7 Receptor Channels
.
Biophysical Journal, 2013, Vol. 104, 12, p. 2612-2621
.
IF = 3.668
[ASEP]
[
doi
]
Bhattacharya, Anirban - Vávra, Vojtěch - Svobodová, Irena - Bendová, Z. - Vereb, G. - Zemková, Hana
.
Potentiation of Inhibitory Synaptic Transmission by Extracellular ATP in Rat Suprachiasmatic Nuclei
.
Journal of Neuroscience, 2013, Vol. 33, 18, p. 8035-8044
.
IF = 6.908
[ASEP]
[
doi
]
Kučka, M. - Kretschmannová, K. - Stojilkovic, S. S. - Zemková, Hana - Tomic, M.
Dependence of spontaneous electrical activity and basal prolactin release on nonselective cation channels in pituitary lactotrophs
.
Physiological Research, 2012, Vol. 61, 3, p. 267-275
.
IF = 1.531
[ASEP]
Jindřichová, Marie - Kuzyk, Pavlo - Li, S. - Stojilkovic, S. S. - Zemková, Hana
.
Conserved ectodomain cysteines are essential for rat P2X7 receptor trafficking
.
Purinergic Signalling, 2012, Vol. 8, 2, p. 317-325
.
IF = 2.635
[ASEP]
[
doi
]
Jindřichová, Marie - Lansdell, S. J. - Millar, N. S.
Changes in Temperature Have Opposing Effects on Current Amplitude in alpha 7 and alpha 4 beta 2 Nicotinic Acetylcholine Receptors
.
PLoS ONE, 2012, Vol. 7, 2, e32073
.
IF = 3.730
[ASEP]
[
doi
]
Zemková, Hana - Stojilkovič, S.S. - Klein, D. C.
Norepinephrine Causes a Biphasic Change in Mammalian Pinealocye Membrane Potential: Role of alfa1B-Adrenoreceptors, Phospholipase C, and Ca2plus
.
Endocrinology, 2011, Vol. 152, 10, p. 3842-3851
.
IF = 4.459
[ASEP]
[
doi
]
Vávra, Vojtěch - Bhattacharya, Anirban - Zemková, Hana
.
Facilitation of glutamate and GABA release by P2X receptor activation in supraoptic neurons from freshly isolated rat brain slices
.
Neuroscience, 2011, Vol. 188, -, p. 1-12
.
IF = 3.380
[ASEP]
[
doi
]
Petrenko, N. - Khafizov, K. - Tvrdoňová, Vendula - Skorinkin, A. - Giniatullin, R.
Role of the Ectodomain Serine 275 in Shaping the Binding Pocket of the ATP-Gated P2X3 Receptor
.
Biochemistry, 2011, Vol. 50, 39, p. 8427-8436
.
IF = 3.422
[ASEP]
[
doi
]
Jindřichová, Marie - Khafizov, K. - Skorinkin, A. - Fayuk, D. - Bart, G. - Zemková, Hana - Giniatullin, R.
Highly conserved tyrosine 37 stabilizes desensitized states and restricts calcium permeability of ATP-gated P2X3 receptor
.
Journal of Neurochemistry, 2011, Vol. 119, 4, p. 676-685
.
IF = 4.061
[ASEP]
[
doi
]
Zemková, Hana - Kučka, M. - Li, S. - Gonzales-Iglesias, A. E. - Tomic, M. - Stojilkovic, S. S.
Characterization of purinergic P2X4 receptor channels expressed in anterior pituitary cells
.
American Journal of Physiology, 2010, Vol. 298, 3, E664-E651
.
IF = 4.686
[ASEP]
[
doi
]
Stojilkovic, S. S. - Yan, Z. - Obšil, T. - Zemková, Hana
.
Structural Insights into the Function of P2X4: An ATP-Gated Cation Channel of Neuroendocrine Cells
.
Cellular and Molecular Neurobiology, 2010, Vol. 30, 8, p. 1251-1258
.
IF = 2.423
[ASEP]
[
doi
]
Stojilkovic, S. S. - He, M. L. - Koshimizu, T. - Balík, A. - Zemková, Hana
.
Signaling by purinergic receptors and channels in the pituitary gland
.
Molecular and Cellular Endocrinology, 2010, Vol. 314, 2, p. 184-191
.
IF = 4.119
[ASEP]
[
doi
]
Rokic, Milos Boro - Tvrdoňová, Vendula - Vávra, Vojtěch - Jindřichová, Marie - Obšil, T. - Stojilkovic, S. S. - Zemková, Hana
.
Roles of conserved ectodomain cysteines of the rat P2X4 purinoreceptor in agonist binding and channel gating
.
Physiological Research, 2010, Vol. 59, 6, p. 927-935
.
IF = 1.646
[ASEP]
Kučka, M. - Kretschmannová, K. - Murano, T. - Wu, Ch. P. - Zemková, Hana - Ambudkar, S. V. - Stojilkovic, S. S.
Dependence of Multidrug Resistance Protein-Mediated Cyclic Nucleotide Efflux on the Background Sodium Conductance
.
Molecular Pharmacology, 2010, Vol. 77, 2, p. 270-279
.
IF = 4.725
[ASEP]
[
doi
]
Jindřichová, Marie - Vávra, Vojtěch - Obšil, Tomáš - Stojilkovic, S. S. - Zemková, Hana
.
Functional relevance of aromatic residues in the first transmembrane domain of P2X receptors
.
Journal of Neurochemistry, 2009, Vol. 109, 3, p. 923-934
.
IF = 3.999
[ASEP]
[
doi
]
Balík, Aleš - Jindřichová, Marie - Bhattacharyya, Sharmistha - Zemková, Hana
.
GnRH-I and GnRH-II-induced calcium signaling and hormone secretion in neonatal rat gonadotrophs
.
Physiological Research, 2009, Vol. 58, 5, p. 709-716
.
IF = 1.430
[ASEP]
Zemková, Hana - Balík, Aleš - Jindřichová, Marie - Vávra, Vojtěch
.
Molecular structure of purinergic P2X receptors and their expression in hypothalamus and pituitary
.
Physiological Research, 2008, Vol. 57, Suppl.3, S23-S38
.
IF = 1.653
[ASEP]
Zemková, H.W. - Bjelobaba, I. - Tomič, M. - Zemková, Hana - Stojilkovic, S. S.
Molecular, pharmacological and functional properties of GABAA receptors in anterior pituitary cells
.
Journal of Physiology, 2008, Vol. 586, 13, p. 3097-3111
.
IF = 4.605
[ASEP]
[
doi
]
Taddei-Ferretti, C. - Radilová, Jiřina - Musio, C. - Santillo, S. - Cibelli, E. - Cotugno, A. - Radil, Tomáš
.
The effects of pattern shape, subliminal stimulation, and voluntary control on multistable visual perception
.
Brain Research, 2008, Vol. 1225, -, p. 163-170
.
IF = 2.494
[ASEP]
[
doi
]
Mazna, Petr - Gryčová, Lenka - Balík, Aleš - Zemková, Hana - Friedlová, Eliška - Obšilová, Veronika - Obšil, Tomáš - Teisinger, Jan
.
The role of proline residues in the structure and function of human MT2 melatonin receptor
.
Journal of Pineal Research, 2008, Vol. 45, 4, p. 361-372
.
IF = 5.056
[ASEP]
[
doi
]
Jelínková, Irena - Vávra, Vojtěch - Jindřichová, Marie - Obšil, Tomáš - Zemková, H.W. - Zemková, Hana - Stojilkovic, S. S.
Identification of P2X4 receptor transmembrane residues contributing to channel gating and interaction with ivermectin
.
Pflugers Archiv - European Journal of Physiology, 2008, Vol. 465, 5, p. 939-950
.
IF = 3.526
[ASEP]
[
doi
]
Zemková, Hana - Yan, Z. - Liang, Z. - Jelínková, Irena - Tomič, M. - Stojilkovic, S. S.
Role of aromatic and charged ectodomain residues in the P2X4 receptor functions
.
Journal of Neurochemistry, 2007, Vol. 102, 4, p. 1139-1150
.
IF = 4.451
[ASEP]
Cellular localization of P2X2 receptors in supraoptic nuclei of hypothalamus Immunohistochemistry on the rat hypothalamic slices containing supraoptic nucleus and optic chiasm (OC). P2X2R immunoreactivity (green) is observed in somata of the supraoptic neurons. Staining was performed using antibodies against the P2X2R (green), neuronal marker NeuN (red) and glia cell marker GFAP (blue). Scale is 50 µm.
