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Analysis of Biologically Important Compounds

Analysis of Biologically Important Compounds Proteins of tooth pulp separated by 2D electrophoresis (excision) and stained with silver.

Main topics:

  • Instrumentation progress in the area of separation methods
  • Methodological progress and development of new separation methods applicable to the estimation of physiologically important compounds (capillary electrophoresis and HPLC/MS).
  • Posttranslational changing of structural proteins (mainly connective tissue)
  • Developing of separation methods for study of proteins and peptides

 

Projects

Achievements

Publications

Ošťádal, M. - Eckhardt, Adam - Herget, J. - Mikšík, Ivan - Dungl, P. - Chomiak, J. - Frydrychová, M. - Burian, M. Proteomic analysis of the extracellular matrix in idiopathic pes equinovarus . Molecular and Cellular Biochemistry. 2015, Vol. 401, 1-2, p. 133-139 . IF = 2.393 [ASEP] [ doi ]
Maurer, G. - Portugal, S. J. - Hauber, M. E. - Mikšík, Ivan - Russell, D. G. D. - Cassey, P. First light for avian embryos: eggshell thickness and pigmentation mediate variation in development and UV exposure in wild bird eggs . Functional Ecology. 2015, Vol. 29, 2, p. 209-218 . IF = 4.828 [ASEP] [ doi ]
Ujčíková, Hana - Eckhardt, Adam - Kagan, Dmytro - Roubalová, Lenka - Svoboda, Petr . Proteomic analysis of post-nuclear supernatant fraction and percoll-purified membranes prepared from brain cortex of rats exposed to increasing doses of morphine . Proteome Science 2014, roč. 12, feb 14, p. 11. ISSN 1477-5956. IF = 1.725 [ASEP] [ doi ]
Mikšík, Ivan - Sedláková, Pavla - Pataridis, Statis - Bortolotti, F. - Gottardo, R. - Tagliaro, F. Prince Cangrande’s Collagen: Study of Protein Modification on the Mummy of the Lord of Verona, Italy (1291–1329 AD) . Chromatographia 2014, roč. 77, 21-22, p. 1503-1510. ISSN 0009-5893. IF = 1.411 [ASEP] [ doi ]
Mikšík, Ivan - Ergang, Peter - Pácha, Jiří . Proteomic analysis of chicken eggshell cuticle membrane layer . Analytical and Bioanalytical Chemistry 2014, roč. 406, 29, p. 7633-7640. ISSN 1618-2642. IF = 3.436 [ASEP] [ doi ]
Jágr, Michal - Eckhardt, Adam - Pataridis, Statis - Broukal, Z. - Dušková, J. - Mikšík, Ivan . Proteomics of human teeth and saliva . Physiological Research 2014, roč. 63, suppl.1, s141-s154. ISSN 0862-8408. IF = 1.293 [ASEP]
Houštěk, Josef - Vrbacký, Marek - Hejzlarová, Kateřina - Zídek, Václav - Landa, Vladimír - Šilhavý, Jan - Šimáková, Miroslava - Mlejnek, Petr - Kazdová, L. - Mikšík, Ivan - Neckář, Jan - Papoušek, František - Kolář, František - Kurtz, T. W. - Pravenec, Michal . Effects of mtDNA in SHR-mtF344 versus SHR conplastic strains on reduced OXPHOS enzyme levels, insulin resistance, cardiac hypertrophy, and systolic dysfunction . Physiological Genomics 2014, roč. 46, 18, p. 671-678. ISSN 1094-8341. IF = 2.374 [ASEP] [ doi ]
Hlaváček, Jan - Vítovcová, M. - Sázelová, Petra - Pícha, Jan - Vaněk, Václav - Buděšínský, Miloš - Jiráček, Jiří - Gillner, D. M. - Holz, R. C. - Mikšík, Ivan - Kašička, Václav . Mono-N-acyl-2,6-diaminopimelic acid derivatives: Analysis by electromigration and spectroscopic methods and examination of enzyme inhibitory activity . Analytical Biochemistry 2014, roč. 467, dec 15, p. 4-13. ISSN 0003-2697. IF = 2.219 [ASEP] [ doi ]
Fargallo, J. A. - López-Rull, I. - Mikšík, Ivan - Eckhardt, Adam - Peralta-Sánchez, J. M. Eggshell pigmentation has no evident effects on offspring viability in common kestrels . Evolutionary Ecology 2014, roč. 28, 4, p. 627-637. ISSN 0269-7653. IF = 2.517 [ASEP] [ doi ]
Eckhardt, Adam - Jágr, Michal - Pataridis, Statis - Mikšík, Ivan . Proteomic Analysis of Human Tooth Pulp: Proteomics of Human Tooth . Journal of Endodontics. 2014, roč. 40, 12, p. 1961-1966. ISSN 0099-2399 . IF = 3.375 [ASEP] [ doi ]

Article photogallery

Capillary electrophoresis: two systems by Beckman (5500 and 5000) with three detectors: UV, DAD and LIF (laser-induced fluorescence) Capillary electrophoresis: two systems by Beckman (5500 and 5000) with three detectors: UV, DAD and LIF (laser-induced fluorescence)
CE-MS coupling (Beckman P/ACE 5000 and Agilent MSD 1100) CE-MS coupling (Beckman P/ACE 5000 and Agilent MSD 1100)
The entire photogallery

Contacts

Institute of Physiology AS CR, v.v.i.
Department of Analysis of Biologically Important Compounds
Vídeňská 1083
14220 Prague 4
tel. +420 241 062 534
fax. +420 241 062 558
 

miksik@fgu.cas.cz

 
 

People

  Prof. Ing. Ivan Mikšík, DrSc.
head of the department
  Mgr. Adam Eckhardt, Ph.D.
senior researcher
  RNDr. Michal Jágr., Ph.D.
senior researcher
  Ing. Jana Svobodová, Ph.D.
senior researcher
  Mgr. Kateřina Lacinová, Ph.D.
senior researcher
  Mgr. Pavla Sedláková, Ph.D.
senior researcher
  Ing. Statis Pataridis
research assistant
  Pavla Šmigolová
lab technician

© 2014 INSTITUTE OF PHYSIOLOGY CAS