Intranet Čeština

Cellular Neurophysiology

Cellular Neurophysiology Electrophysiological recording of glutamate-induced current responses in cultured hippocampal neurons.

Our research is mainly directed to exploration of functional and pharmacological properties of ion channels, including those activated during synaptic transmission. For that purpose, we use electrophysiological methods, primarily patch clamp technique, combined with molecular-biology, immunohistochemical and microfluorometric methods (Ca2+ imaging). Using this approach, we study properties of excitable cells, such as cultured hippocampal and granular neurons or in vitro preserved neural circuits of pyramidal neurons in the hippocampal brain slices, as well as HEK293 and COS cell lines transfected with recombinant NMDA or vanilloid receptors. The goal of our research is to comprehend the relationship between structure and function of ion channels, to characterize the actions of pharmacologically active substances and to explain the molecular mechanism by which they influence ligand-gated ion channels.

For more detailed understanding of physiological mechanisms and pharmacology of ligand-gated ion channels, we use quantitative analytical techniques (including the analysis of single ion channels, measuring of intracellular concentrations of Ca2+ and mathematical modeling).

 

For more information and current projects visit department´s web.

Publications

Zíma, V. - Witschas, Katja - Hynková, Anna - Zímová, Lucie - Barvík, I. - Vlachová, Viktorie . Structural modeling and patch-clamp analysis of pain-related mutation TRPA1-N855S reveal inter-subunit salt bridges stabilizing the channel open state . Neuropharmacology. 2015, Vol. 93, Jun, p. 294-307 . IF = 5.106 [ASEP] [ doi ]
Witschas, Katja - Jobin, M.-L. - Korkut, D. N. - Vladan, M. M. - Salgado, G. - Lecomte, S. - Vlachová, Viktorie - Alves, I. D. Interaction of a peptide derived from C-terminus of human TRPA1 channel with model membranes mimicking the inner leaflet of the plasma membrane . Biochimica Et Biophysica Acta-Biomembranes. 2015, Vol. 1848, 5, p. 1147-1159 . IF = 3.836 [ASEP] [ doi ]
Vyklický, Vojtěch - Krausová, Barbora - Černý, Jiří - Balík, Aleš - Zápotocký, Martin - Novotný, M. - Lichnerová, Katarina - Smejkalová, Tereza - Kaniaková, Martina - Kořínek, Miloslav - Petrovič, Miloš - Kačer, P. - Horák, Martin - Chodounská, Hana - Vyklický ml., Ladislav . Block of NMDA receptor channels by endogenous neurosteroids: implications for the agonist induced conformational states of the channel vestibule . Scientific Reports. 2015, Vol. 5, Jun 18, p. 10935 . IF = 5.578 [ASEP] [ doi ]
Šepsová, V. - Karasová, J. Z. - Tobin, G. - Jun, D. - Korábečný, J. - Cabelová, P. - Jánská, K. - Krůšek, Jan - Skřenková, K. - Kuča, M. - Valko, M. - Soukup, O. Cholinergic properties of new 7-methoxytacrine-donepezil derivatives . General Physiology and Biophysics. 2015, Vol. 34, 2, p. 189-200 . IF = 1.173 [ASEP] [ doi ]
Lichnerová, Katarina - Kaniaková, Martina - Park, S. P. - Skřenková, Kristýna - Wang, Y.- X. - Petralia, R. S. - Suh, Y. H. - Horák, Martin . Two N-glycosylation Sites in the GluN1 Subunit Are Essential for Releasing N-methyl-D-aspartate (NMDA) Receptors from the Endoplasmic Reticulum . Journal of Biological Chemistry. 2015, Vol. 290, 30, p. 18379-18390 . IF = 4.573 [ASEP] [ doi ]
Kudová, Eva - Chodounská, Hana - Slavíková, Barbora - Buděšínský, Miloš - Nekardová, Michaela - Vyklický, Vojtěch - Krausová, Barbora - Švehla, Pavel - Vyklický ml., Ladislav . A New Class of Potent N-Methyl-D-Aspartate Receptor Inhibitors: Sulfated Neuroactive Steroids with Lipophilic D-Ring Modifications . Journal of Medicinal Chemistry. 2015, Vol. 58, 15, p. 5950-5966 . IF = 5.447 [ASEP] [ doi ]
Kořínek, Miloslav - Vyklický, Vojtěch - Borovská, Jiřina - Lichnerová, Katarina - Kaniaková, Martina - Krausová, Barbora - Krůšek, Jan - Balík, Aleš - Smejkalová, Tereza - Horák, Martin - Vyklický ml., Ladislav . Cholesterol modulates open probability and desensitization of NMDA receptors . Journal of Physiology. 2015, Vol. 593, 10, p. 2279-2293 . IF = 5.037 [ASEP] [ doi ]
Boušová, Kristýna - Jirků, Michaela - Bumba, Ladislav - Bednárová, Lucie - Šulc, Miroslav - Franěk, M. - Vyklický ml., Ladislav - Vondrášek, Jiří - Teisinger, Jan . PIP2 and PIP3 interact with N-terminus region of TRPM4 channel . Biophysical Chemistry. 2015, Vol. 205, Oct 2015, p. 24-32 . IF = 1.986 [ASEP] [ doi ]
Vyklický, Vojtěch - Kořínek, Miloslav - Smejkalová, Tereza - Balík, Aleš - Krausová, Barbora - Kaniaková, Martina - Lichnerová, Katarina - Černý, Jiří - Krůšek, Jan - Dittert, Ivan - Horák, Martin - Vyklický ml., Ladislav . Structure, function, and pharmacology of NMDA receptor channels . Physiological Research 2014, roč. 63, suppl.1, s191-s203. ISSN 0862-8408. IF = 1.293 [ASEP]
Šepsová, V. - Krůšek, Jan - Zdarová Karasová, J. - Zemek, F. - Musílek, K. - Kuča, K. - Soukup, O. The interaction of quaternary reversible acetylcholinesterase inhibitors with the nicotinic receptor . Physiological Research. 2014, Vol. 63, 6, p. 771-777 . IF = 1.293 [ASEP]

Article photogallery

Contacts

Institute of Physiology AS CR, v.v.i.
Department of Cellular Neurophysiology

Vídeňská 1083
14220 Prague 4
tel. +420 241 062 450
fax. +420 241 062 488
 

vyklicky@fgu.cas.cz

People

Doc. MUDr. Ladislav Vyklický, DrSc.
head of the department
RNDr. Aleš Balík, Ph.D.
senior researcher
Ing. Ivan Dittert, Ph.D.
senior researcher
Mgr. Martin Horák, Ph.D.
senior researcher
Mgr. Miloslav Kořínek, Ph.D.
vědecký pracovník
RNDr. Jan Krůšek, Ph.D.
senior researcher
RNDr. Viktorie Vlachová, DrSc.
vědecký pracovník
Prof. RNDr. František Vyskočil, DrSc.
senior researcher
Mgr. Martina Kaniaková, Ph.D.
postdoktorand
RNDr. Lenka Maršáková, Ph.D.
postdoktorand
Tereza Smejkalová, Ph.D.
postdoktorand
RNDr. Vojtěch Vyklický, Ph.D.
postdoktorand
Mgr. Lucie Zímová, Ph.D.
postdoktorand
Mgr. Anna Hynková
PhD student
Mgr. Barbora Krausová
PhD student
Mgr. Katarina Lichnerová
PhD student
Mgr. Filip Touška
PhD student
Mgr. Marek Ladislav
PhD student
Ing. Pavla Hubálková
PhD student
Mgr. Kristýna Skřenková
PhD student
Jiří Kaucký
technician
Miloslava Kuldová
technician
Magda Kuntošová
technician

© 2014 INSTITUTE OF PHYSIOLOGY CAS