Tomáš Špringer, Ph.D.

Research interests:

Study of nucleic acids and their analogues significant in therapies based on suppression of genetic expression.

Development of microfluidics for SPR biosensors.

Application of gold nanoparticles for ultra-sensitive SPR biosensors.

Surface plasmon resonance (SPR) biosensors.

Education:

2006 - today: PhD study, Charles University, Faculty of Mathematics and Physics (Biophysics) at the Institute of Photonics and Electronics, dpt. of Optical Sensors

Thesis: In situ study of interactions of nucleic acids significant in genetic expression and therapy based on its suppression
Supervisor: Prof. Josef Stepanek, PhD.

2001 - 2006: Master of Science, Charles University, Faculty of Mathematics and Physics (Biophysics)

Thesis: Surface-enhanced photophysical processes of organic molecules in metal nanoparticles
Supervisor: Doc. RNDr. Marek Procházka, Dr.

Awards:

2^nd poster award, Europt(r)ode X 2010, Prague.

Publications:

Book chapters:

1. P. Adam, M. Piliarik, H. Šípová, T. Špringer, M. Vala, J. Homola: Surface Plasmons for Biodetection, in Photonic Sensing: Principles and Applications for Safety and Security Monitoring, editors W. J. Bock, J., X. Gaozhi, John Wiley & Sons, 2012, 1-58.

Journal papers:

1. M. Bocková, T. Špringer, I. Nečasová, J. Nunvar, B. Schneider, and J. Homola: Monitoring RAYT activity by surface plasmon resonance biosensor, Analytical and Bioanalytical Chemistry, 407, 3985-3993 (2015).
2. H. Vaisocherová, I. Víšová, M. Bocková, T. Špringer, M. L. Ermini, X. Song, Z. Krejčík, L. Chrastinová, O. Pastva, K. Pimková, M. Dostálová Merkerová, J. E. Dyr, and J. Homola: Rapid and sensitive detection of multiple MicroRNAs in cell lysate by low-fouling surface plasmon resonance biosensor, Biosensors and Bioelectronics, 70, 226–231 (2015).
3. T. Špringer, M. L. Ermini, B. Špačková, J. Jabloňků, J. Homola: Enhancing sensitivity of SPR biosensors by functionalized gold nanoparticles: size matters, Analytical Chemistry, 86, 10350−10356 (2014)
4. H. Šípová, T. Špringer, D. Rejman, O. Šimák, M. Petrová, P. Novák, Š. Rosenbergová, O. Páv, R. Liboska, I. Barvík, J. Štěpánek, I. Rosenberg, J. Homola: 5’-O-Methylphosphonate nucleic acids (MEPNA) – new modified DNAs that increase the E. coli RNase H cleavage rate of hybrid duplexes, Nucleic Acids Research, 42, 5378–5389 (2014). link
5. T. Špringer, M. Bocková, J. Homola: Label-Free Biosensing in Complex Media: A Referencing Approach.  Analytical Chemistry, 85 (2013), 5637–5640. link
6. T. Špringer, J. Homola: Biofunctionalized gold nanoparticles for SPR-biosensor-based detection of CEA in blood plasma. Analytical and Bioanalytical Chemistry, 404 (2012), 2869-2875. link
7. H. Šípová, T. Špringer, J. Homola: Streptavidin-enhanced assay for sensitive and specific detection of single nucleotide polymorphism in TP53. Analytical and Bioanalytical Chemistry, 399 (2011), 2343-2350. link
8. T. Špringer, H. Šípová, H. Vaisocherová, J. Štepánek, J. Homola: Shielding effect of monovalent and divalent cations on solid-phase DNA hybridization: surface plasmon resonance study. Nucleic Acids Research, 38 (2010), 7343-7351. link
9. T. Špringer, M. Piliarik, J. Homola: Surface plasmon resonance sensor with dispersionless microfluidics for direct detection of nucleic acids at the low femtomole level. Sensors and Actuators B - Chemical, 145 (2010), 588-591. link
10. T. Špringer, M. Piliarik, J. Homola: Real-time monitoring of biomolecular interactions in blood plasma using a surface plasmon resonance biosensor. Analytical and Bioanalytical Chemistry, 398 (2010),1955-1961. link
11. H. Vaisocherová, J. Snášel, T. Špringer, H. Šípová, I.  Rosenberg, J. Štěpánek, J. Homola: Surface plasmon resonance study on HIV-1 integrase strand transfer activity. Analytical and Bioanalytical Chemistry, 393 (2009), 1165–1172. link

Conference papers:

1. T. Špringer, M. L. Ermini, P. Kvasnička, B. Špačková, J. Homola: Size effect of neutravidin-coated gold nanoparticles on the enhanced SPR sensor response in the detection of carcinoembryonic antigen. Advances in Biodetection and Biosensors 2014, Berlin.
2. T. Špringer, J. Homola: Functionalized nanoparticles for the amplification of sensor response in SPR biosensors. Europt(r)ode XI 2012, Barcelona.
3. P. Kvasnička, T. Špringer, J. Homola: Single-DNA binding event detection in amplified micro-SPR surface plasmon resonance sensor. Europt(r)ode XI 2012, Barcelona.
4. T. Špringer, M. Piliarik, J. Homola: Real-time monitoring of biomolecular interactions in blood plasma using surface plasmon resonance sensor. Europt(r)ode X 2010, Prague.
5. H. Šípová, T. Špringer, J. Homola: Streptavidin-enhanced assay for sensitive and specific detection of single nucleotide polymorphism in TP53. Europt(r)ode X 2010, Prague.
6. M. Vala, H. Šípová, T. Špringer, K. Chadt, M. Piliarik, J. Homola: Compact multi-channel surface plasmon resonance sensor for rapid detection of bacterial pathogens. Europt(r)ode X 2010, Prague.