SR7500DC SPR System
Location: |
Research team of Dr. Jan Konvalinka |
Responsible person: |
Dr. Milan Kožíšek (☎ 220 183 518) |
SR7500DC Surface Plasmon Resonance System (Reichert Instruments) is the last generation of surface plasmon resonance (SPR) technology with
incomparable sensitivity and high-quality binding data for interaction analysis - all in a flexible component-based platform. The key to the system
is the advanced SR7500 Dual Channel SPR Spectrometer instrument. The label-free system generates data on critical interactions between proteins,
nucleic acids, lipids, carbohydrates, small molecules, whole cells, bacteria, viruses, and polymers.
Components of the SR7500DC System include:
- SR7500DC apectrometer
- autosampler
- programmable syringe pump
- diverter valve
- flow cells
- sensor chips
Metrohm Autolab
Location: |
Research team of Prof. Josef Michl |
Responsible person: |
Dr. Jiří Kaleta (☎ 220 183 342) |
The depicted instrumental assembly is used for cyclic voltammetry and similar electrochemical measurements. This is essential for
studies of monolayers and molecular grids for mounting molecular rotors. The key part of the system are Metrohm 663 VA Stand,
Metrohm Autolab Potentiostat/Galvanostat and Tektronix TDS 1002B digital oscillosope.
SPECTRO Arcos
Location: |
Analytical Laboratory |
Responsible person: |
Stanislava Matějková (☎ 220 183 118) |
SPECTRO Arcos (SPECTRO Analytical Instruments, Kleve, Germany) is an optical emission spectrometer (OES)
with radial observation of inductively coupled plasma (ICP). The SPECTRO ARCOS features a Paschen-Runge spectrometer mount,
employing the Optimized Rowland Circle Alignment (ORCA) technique with 32 linear CCD detectors. The optics is
hermetically sealed and filled with argon. Thus, high optical transmission in the VUV is achieved, allowing the
determination of non-metals as well as the use of prominent and interference free lines in this region.
The wavelength range between 130 and 770 nm can be simultaneously analyzed, allowing complete spectrum capture
within 2s. Hence, a new measurement is not required even if additional elements or lines are to be determined at a
later date. All relevant ICP OES operating parameters are software controlled, allowing easy selection of the optimum
operating conditions.
PE 2400 Series II CHN Analyzer
Location: |
Analytical Laboratory |
Responsible person: |
Stanislava Matějková (☎ 220 183 118) |
The PE 2400 Series II CHNS/O Analyzer (Perkin Elmer, USA) serves for simultaneous determination of C, H, and N in organic compounds.
In the CHN operating mode the instrument employs a classical combustion principle to convert the sample elements to simple gases (CO2, H2O and N2).
The PE 2400 analyzer performs automatically combustion and reduction, homogenization of product gases, separation and detection. A microbalance MX5 (Mettler Toledo) is used for precise weighing of samples.
The accuracy of CHN determination is better than 0.30% abs.
AUTOPOL IV
Location: |
Analytical Laboratory |
Responsible person: |
Stanislava Matějková (☎ 220 183 118) |
The AUTOPOL IV (Rudolph Research Analytical, USA) is a microprocessor based automatic polarimeter for measurement of optical rotation (±89° Arc), specific rotation ±999.99° Arc)
and concentration (0 - 99.9%). The Autopol IV comprises Glan Thomson calcite quartz prism polarizer. The highest resolution is 0.001° Arc for optical rotation, 0.001° below 90° Arc and 0.01°
between 100 to 999.99° Arc for specific rotation and 0.001% for concentration. However, usually the resulting values of specific rotation are given with one decimal place.
The polarimeter accepts sample cells up to 200 mm. The instrument was installed in 2001.
SPECTRO iQ II
Location: |
Analytical Laboratory |
Responsible person: |
Stanislava Matějková (☎ 220 183 118) |
The simultaneous energy-dispersive X-ray fluorescence benchtop spectrometer SPECTRO iQ II
(SPECTRO Analytical Instruments, Kleve, Germany) was purchased in 2008. The samples are excited by a forced air-cooled
low-power Pd end-window X-ray tube combined with a doubly curved HOPG crystal for monochromatization and polarization
of the primary tube radiation. A VITUS-Silicon Drift Detector (SDD) with Ta collimator is used to collect the
fluorescence emission from the sample. The resolution of the SDD is better than 160 eV for Mn Kα. All radiation
paths are flushed with helium. The measurement parameters are controlled by the system PC. The SPECTRO iQ II
spectrometer allows a non-destructive simultaneous multi-element both qualitative and (after development of calibration
method) quantitative analysis of elements from Na to U in different types of sample forms (except gases).
Zetasizer Nano ZS
Location: |
Research Team of Dr. Petr Cígler |
Responsible person: |
Dr. Petr Cígler (☎ 220 183 429) |
Zetasiser Nano ZS analyzer is a highly effective, yet simple and versatile system for the enhanced detection of aggregates and measurement
of small or dilute samples, and samples at very low or high concentration using dynamic light scattering with 'NIBS' optics. It measures the
particle size (0.6 nm to 8.9 micron range), zeta potential of colloids and nanoparticles, the molecular weight of random coiled polymers up to 5×105 Da as
well as globular polymers and proteins up to 2×107 Da, and optionally the measurement of protein mobility and microrheology of protein and polymer solutions.
Agilent Bravo
Location: |
Biochemical Pharmacology of Antimetabolites (Dr. Helena Mertlíková-Kaiserová) |
Responsible person: |
Pavla Plačková (☎ 220 183 528) |
Agilent Bravo Automated Liquid Handling Platform is both versatile and precise, loaded into a small, compact footprint. It is the fastest, most versatile liquid-handling system available.
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