Curriculum vitae:
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1995-2000
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master’s level studies at the Institute of Chemical Technology, Prague. Field: biochemistry
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1998-2000
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Graduate research student at IAPG CAS, Prague
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2000-2006
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Ph.D. studies at Institute of Chemical Technology, Prague. Field: microbiology
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2000-2006
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postgraduate researcher at IAPG CAS, Prague
PhD thesis: Ecology of butyrate producing bacteria
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2006-2010
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postdoctoral researcher at IAPG CAS, Prague
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2010-present
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staff researcher at IAPG CAS, Prague
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Specialization:
Main area of interest is the study of bacterial, archeal and fungal diversity of human and animal digestive tract. Research topics include microbiome description of patients with inflammation diseases (Crohns disease, ulcerative colitis, primary sclerosing cholangitis), therapy influence (fecal and liver transplantation) and also influence of food additives (chitosan) on human microbiome. Another research topic is based on nutrition studies of farm animals, where we monitor the changes of intestinal microflora under different diet conditions. We have also recently focused on description of biowaste microbiome with aim to improve biogas production by modifying microbiome composition. Another research topis is the description of new bacterial and fungal taxons and their phylogenetic affiliation.
To solve these topics we use methods of molecular biology: PCR-DGGE, quantitative PCR, Sanger sequencing and next-generation sequencing on Ion Torrent platform.
Societies:
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Czechoslovak Society for Microbiology |
Internship abroad:
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2001-2002
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Slovak Academy of Sciences, IAP, Košice, Slovak republic
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2002
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University of Ljubljana, Faculty of Biotechnology, Domžale, Slovenia
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2003-2003
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Rowett Research Institute, Aberdeen, UK
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2008
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Aberystwyth University, Aberystwyth, UK
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Foreign cooperation
Ljubljana University, Domžale, Slovinia: taxonomy of butarate-producing bacteria
Slovak academy of sciences, ÚFHG, Košice, Slovakia: restriction endonucleases of butyrate producing bacteria
Rowet Research Institute, Aberdeen, GB: natural transformation of anaerobic bacteria
Aberystwyth University, Wales: taxonom of anaerobic fungi
Innsbruck University, Austria: biogas production
Mendoza Univerzita, Argentina: analysis of goat digestive tract microbiom
Publications:
| 2003 |
Kopečný J, Marinšek-Logar R, Zorec M, Mrázek J, Kobayashi Y (2003) Butyrivibrio hungatei sp. nov., and Pseudobutyrivibrio xylanivorans sp. nov., butyrate-producing bacteria from the rumen. Int J Syst Evol Micr 53 (1): 201-209
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| 2005 |
Mrázek J., Piknová M., Pristaš P., Kopečný J.: Occurence of restriction-modification systems in ruminal butyrate-producing bacteria. Anaerobe 11:280-284. |
| 2008 |
Scott K.P., Martin J.C., Mrazek J., Flint H.J.: Transfer of conjugative elements from rumen and human Firmicutes bakteria to Roseburia inulinivorans. Appl Environ Microbiol. 74 (12):3915-3917. |
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Mrázek J., Štrosová L., Fliegerová K., Kott T., Kopečný J.: Diversity of Insect Intestinal Microflora. Folia Microbiol. 53 (3):229-233. |
| 2009 |
Killer J, Kopecny J, Mrázek J,Rada V, Benada O, Koppová I, Havlík J, Straka J (2009) Bifidobacterium bombi sp. nov., from the bumblebee digestive tract. Int J Syst Evol Micr 59 (8): 2020-2024
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| 2010 |
Mrázek J., Koppová I., Kopečný J., Šimůnek J., Fliegerová K.: PCR-DGGE-based study of fecal microbial stability during the long-term chitosan supplementation of humans. Folia Microbiol. 55 (4):352-358. |
| 2011 |
Zakostelska Z, Kverka M, Klimesova K, Rossman P, Mrazek J, Kopecny J, Hornova M, Srutkova D, Hudcovic D, Ridl J, Tlaskalova-Hogenova H (2011) Lysate of probiotic Lactobacillus casei DN-114 001 ameliorates colitis by strengthening the gut barrier function and changing the gut microenvironment. PLoS ONE 6 (11) , art. no. e27961
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Kverka M., Zakostelska Z., Klimesova K., Sokol D., Hudcovic T., Hrncir T., Rossmann P., Mrazek J., Kopecny J., Verdu E.F., Tlaskalova-Hogenova H.: Oral administration of Parabacteroides distasonis antigens attenuates experimental murine colitis through modulation of immunity and microbiota composition. Clinical and Experimental Immunology. 163 (2):250-259. |
| 2012 |
Vlčková K, Mrázek J, Kopečný J, Petrželková KJ (2012) Evaluation of different storage methods to characterize the fecal bacterial communities of captive western lowland gorillas (Gorilla Gorilla Gorilla). J Microbiol Methods 91 (1): 45-51
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Prochazka J, Fliegerová K, Mrázek J, Štrosová L, Zábranská J, Dohányos M (2012) Enhanced biogas yield from energy crops with rumen anaerobic fungi. Eng Life Sci 12 (3): 343-351
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Michiels J, Skrivanova E, Missotten J, Ovyn A, Mrazek J, De Smet S, Dierick N (2012) Intact brown seaweed (Ascophyllum nodosum) in diets of weaned piglets: effects on performance, gut bacteria and morphology and plasma oxidative status. J Anim Phys Anim Nutr 96 (6): 1101-1111
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Čermáková J, Mrázek J, Fliegerová K, Tenkrát D (2012) Replacing natural gas by biogas - determining the bacterial contamination of biogas by PCR. Acta Polytech 52 (4): 33-38
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| 2013 |
Klimesova K, Kverka M, Zakostelska Z, Hudcovic T, Hrncir T, Stepanova R, Rossmann P, Ridl J, Kostovcik M, Mrazek J, Kopecny J, Kobayashi KS, Tlaskalova-Hogenova H (2013) Altered gut microbiota promotes colitis-associated cancer in IL-1 receptor-associated kinase M-deficient mice. Inflam Bowel Dis. 19 (6): 1266-1277
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| 2014 |
Fliegerova K, Tapio I, Bonin A, Mrazek J, Callegari ML, Bani P, Bayat A, Vilkki J, Kopecny J, Shingfield KJ, Boyer F, Coissac E, Taberlet P, Wallace JR (2014) Effect of DNA extraction and sample preservation method on rumen bacterial population. Anaerobe 29: 80-84
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Pechar R, Rada V, Parafati L, Musilova S, Bunesova V, Vlkova E, Mrazek J, Kmet V, Svejstil R (2014) Mupirocin-mucin agar for selective enumeration of Bifidobacterium bifidum. Int J Food Microbiol 191: 32-35
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