The aim of the project is quantitave evaluation of length density and spatial arrangement of brain microvessels from stacks of optical sections captured by a laser scanning confocal microscope. We will use image enhancement by deconvolution, horizontal and vertical image composition of adjacent fields of view. We will develop and test several methods for capillary detection, measure the length automatically and compare the results with state of art interactive techniques of length measurement. The detection methods will include use of top-hat filters, path extraction methods by minimal path and probabilistic methods using Markov random fields of directions. Spatial arrangement will be characterized by topology, correlation functions, sphere contact distribution function and rose of directions of microvessels.
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