Hocek Research Team

Ing. Hana Macíčková-Cahová, Ph.D.

e-mail: cahova@uochb.cas.cz
phone: +420 220183343

Born 27. 12. 1981 in Zlín (Czech Republic).

Scientific CV:
2001-2006 undergraduate study, Prague Institute of Chemical Technology, M. S. degree - specialization organic chemistry (supervisor Associate Prof. I. Linhart)
2005 study abroad for 4 months ENSCM, Montpellier, France (group of prof. J. Moreau, supervisor P. Hesemann)
2006-2010 PhD study, Institute of Organic Chemistry and Biochemistry, Academy of Sciences of the Czech Republic (supervisor Dr. M. Hocek)
2010-2013 Postdoctoral stay, University of Heidelberg, Jäschke's Group
since 2014 Postdoctoral position, Institute of Organic Chemistry and Biochemistry, Academy of Sciences of the Czech Republic (supervisor Dr. M. Hocek)

Awards:
2008 Best oral presentation - YoungChem 2008, Cracow, Poland
2009 Best oral presentation - The Progress in Organic, Bioorganic and Pharmaceutical Chemistry 2009, Nymburk, Czech Republic
2010 Best oral presentation – IOCB Conference 2010, Frymburk, Czech Republic
2012 Best poster presentation – American Chemical Society prize - EMBO conference series – Chemical Biology 2012, Heidelberg, Germany

List of publications:

17.	Cahová, H.; Panattoni, A.; Kielkowski, P.; Fanfrlík, J.; Hocek, M.: "5-Substituted Pyrimidine and 7-Substituted 7-Deazapurine dNTPs as Substrates for DNA Polymerases in Competitive Primer Extension in the Presence of Natural dNTPs" ACS Chem. Biol. 2016, 11, 3165 - 3171.
16.	Dadová, J.; Cahová, H.; Hocek, M.: "Polymerase Synthesis of Base-Modified DNA" in K. Nakatani, Y. Tor (eds.), Modified Nucleic Acids, Nucleic Acids and Molecular Biology Series, Vol. 31, Springer 2016, 123 - 144.
15.	Kielkowski, P.; Cahová, H.; Pohl, R.; Hocek, M.: "Flexible double-headed cytosine-linked 2'-deoxycytidine nucleotides. Synthesis, polymerase incorporation to DNA and interaction with DNA methyltransferases" Bioorg. Med. Chem. 2016, 24, 1268 – 1276.
14.	Cahová, H.; Winz M.-L.; Höfer K., Nübel G.; Jäschke A.: "NAD captureSeq indicates NAD as a bacterial cap for a subset of regulatory RNAs" Nature 2015, 519, 374-377.
13.	Cahová, H.; Jäschke A.: "Nucleoside-Based Diarylethene Photoswitches and Their Facile Incorporation into Photoswitchable DNA" Angew. Chem. Int. Ed. 2013, 52, 3186-3190.
12.	Ménová, P.; Cahová, H.; Plucnara, M.; Havran, L.; Fojta, M.; Hocek, M.: "Polymerase synthesis of oligonucleotides containing a single chemically modified nucleobase for site-specific redox labelling" Chem. Commun. 2013, 49, 4652-4654.
11.	Kielkowski, P.; Macíčková-Cahová, H.; Pohl, R.; Hocek, M.: "Transient and Switchable (Triethylsilyl)ethynyl Protection of DNA against Cleavage by Restriction Endonucleases" Angew. Chem. Int. Ed. 2011, 50, 8727 –8730.
10.	Macíčková-Cahová, H.; Pohl, R.; Horáková, P.; Havran, L.; Špaček, J.; Fojta, M.; Hocek, M.: "Alkylsulfanylphenyl Derivatives of Cytosine and 7-Deazaadenine Nucleosides, Nucleotides and Nucleoside Triphosphates: Synthesis, Polymerase Incorporation to DNA and Electrochemical Study" Chem. Eur. J. 2011, 17, 5833–5841.
9.	Horáková, P.; Macíčková-Cahová, H.; Pivoňková, H.; Špaček, J.; Havran, L.; Hocek, M.; Fojta, M.: "Tail-labelling of DNA probes using modified deoxynucleotide triphosphates and terminal deoxynucleotidyl tranferase. Application in electrochemical DNA hybridization and protein-DNA binding assays" Org. Biomol. Chem. 2011, 9, 1366 – 1371.
8.	Macíčková-Cahová, H.; Pohl, R.; Hocek, M.: "Cleavage of Functionalized DNA Containing 5-Modified Pyrimidines by Type II Restriction Endonucleases" ChemBioChem 2011, 12, 431 – 438.
7.	Macíčková-Cahová, H.; Vrábel, M.; Hocek, M.: "Cross-Coupling Modification of Nucleoside Triphosphates, PEX, and PCR Construction of Base-Modified DNA" Curr. Protoc. Chem Biol. 2010, 2, 1-14.
6.	Ikonen, S.; Macíčková-Cahová, H.; Pohl, R.; Šanda, M.; Hocek, M.: "Synthesis of nucleoside and nucleotide conjugates of bile acids, and polymerase construction of bile acid-functionalized DNA" Org. Biomol. Chem. 2010, 8, 1194-1201.
5.	Macíčková-Cahová, H.; Hocek, M.: "Cleavage of adenine-modified functionalized DNA by type II restriction endonucleases" Nucleic Acids Res. 2009, 37, 7612–7622.
4.	Vrábel, M.; Horáková, P.; Pivoňková, H.; Kalachova, L.; Černocká, H.; Cahová, H.; Pohl, R.; Šebest, P.; Havran, L.; Hocek, M.; Fojta, M.: "Base-Modified DNA Labeled by [Ru(bpy)₃]²⁺ and [Os(bpy)₃]²⁺ Complexes: Construction by Polymerase Incorporation of Modified Nucleoside Triphosphates, Electrochemical and Luminescent Properties, and Applications" Chem. Eur. J. 2009, 15, 1144-1154.
3.	Cahová, H.; Pohl, R.; Bednárová, L.; Nováková, K.; Cvačka, J.; Hocek, M.: "Synthesis of 8-bromo-, 8-methyl- and 8-phenyl-dATP and their polymerase incorporation into DNA" Org. Biomol. Chem. 2008, 6, 3657-3660.
2.	Cahová, H.; Havran, L.; Brázdilová, P.; Pivoňková, H.; Pohl, R.; Fojta, M.; Hocek, M.: "Aminophenyl- and Nitrophenyl-Labeled Nucleoside Triphosphates: Synthesis, Enzymatic Incorporation, and Electrochemical Detection" Angew. Chem. Int. Ed. 2008, 47, 2059–2062.
1.	Čapek, P.; Cahová, H.; Pohl, R.; Hocek, M.; Gloeckner, C.; Marx, A.: "An Efficient Method for the Construction of Functionalized DNA Bearing Amino Acid Groups through Cross-Coupling Reactions of Nucleoside Triphosphates Followed by Primer Extension or PCR" Chem. Eur. J. 2007, 13, 6196–6203.