Čeština
EnglishTMV-RNA biosynthesis in the light-green and dark-green regions of tobacco leaves
Šindelářová, Milada; Šindelář, Luděk
BIOLOGIA PLANTARUM 48 [3]: 419-423, 2004
Keywords: oxidative pentosephosphate cycle; ribonuclease; phosphodiesterase
Abstract: Changes in the proteins, chlorophyll, virus content and activity of key enzymes of viral RNA biosynthesis wereinvestigated in the light- and dark-green regions of tobacco leaves systemically infected with tobacco mosaic virus. The comparative analysis of soluble proteins from healthy and light- or darkgreen regions of leaves revealed that the main soluble proteins are equal in pattern but differ inquantity. The contents of chlorophylls did not differ from healthy tissues in the dark-green islands but were considerably lower in the light-green regions. The content of virus in light-green tissues was about 10 times higher than in the darkgreen islands. The activities of key enzymes of oxidative pentosephosphate cycle did not differ from healthy tissues in the dark-green islands but were considerably higher in the light-green tissues when compared with healthy control. Similar relationships were observed for ribonuclease, phosphomonoesterase and phosphodiesterase activities.
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IEB authors:
BIOLOGIA PLANTARUM 48 [3]: 419-423, 2004
Keywords: oxidative pentosephosphate cycle; ribonuclease; phosphodiesterase
Abstract: Changes in the proteins, chlorophyll, virus content and activity of key enzymes of viral RNA biosynthesis wereinvestigated in the light- and dark-green regions of tobacco leaves systemically infected with tobacco mosaic virus. The comparative analysis of soluble proteins from healthy and light- or darkgreen regions of leaves revealed that the main soluble proteins are equal in pattern but differ inquantity. The contents of chlorophylls did not differ from healthy tissues in the dark-green islands but were considerably lower in the light-green regions. The content of virus in light-green tissues was about 10 times higher than in the darkgreen islands. The activities of key enzymes of oxidative pentosephosphate cycle did not differ from healthy tissues in the dark-green islands but were considerably higher in the light-green tissues when compared with healthy control. Similar relationships were observed for ribonuclease, phosphomonoesterase and phosphodiesterase activities.
DOI: