Recording of electrophysiological activity from dorsal horn neurons in acute spinal cord slices with patch-clamp technique.
The main research interest of our department is to study mechanisms of pain and to explore new possibilities of pain treatment, especially in chronic states. Our experimental work is concentrated on the modulation of nociceptive information at the spinal cord level that is the relay center between the periphery and the higher brain areas. Our goal is to study these modulatory mechanisms in order to improve therapy for pain conditions, such as allodynia, hyperalgesia, neuropathic and cancer related pain. Our focus is on the role of TRPV1 receptors and inflammatory cytokines. In our research we use mainly electrophysiological, immunohistochemical and behavioral methods. In addition, we finalize another project directed towards the role of thyroid hormones (THs) and omega-3-polyunsaturated fatty acid (n-3 PUFA) in the physiology of cardiac and skeletal muscles. Models of hyper- and hypothyroid status are used to analyze potential influence of n-3 PUFA on the induced pathological changes.
Projects
Scientists from the Department of Functional Morphology in collaboration with colleagues from The University of Texas showed that paclitaxel induced pain is mediated by TRPV1 and TLR4 receptors.
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N-acylphosphatidylethanolamine (NAPE), as the precursor of anandamide, an endogenous agonist of TRPV1 and cannabinoid receptors, could play a role in modulation of nociceptive signalization in the spinal cord.
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Achievements
Aktualne.TV - interview, 18.3. 2015
The interview with Dr. Paleček about the research whose aim is to eliminate chronic pain.
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Altogether 70 scientists from 17 foreign countries and 6 from the Czech Republic have attended the meeting as registered participants.
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Publications
Mrózková, Petra - Paleček, Jiří - Špicarová, Diana
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The role of protease-activated receptor type 2 in nociceptive signaling and pain
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Physiological Research. 2016, roč. 65, 3, p. 357-367
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IF = 1.643
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Molet, J. - Mauborgne, A. - Diallo, Michael - Armand, V. - Geny, D. - Villanueva, L. - Boucher, Y. - Pohl, M.
Microglial Janus kinase/signal transduction and activator of transcription 3 pathway activity directly impacts astrocyte and spinal neuron characteristics
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Journal of Neurochemistry. 2016, roč. 136, 1, p. 133-147
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IF = 3.842
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Mrózková, Petra - Špicarová, Diana - Paleček, Jiří
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Hypersensitivity Induced by Activation of Spinal Cord PAR2 Receptors Is Partially Mediated by TRPV1 Receptors
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PLoS ONE. 2016, roč. 11, 10, článku e0163991
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IF = 3.057
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Szeiffová Bačová, B. - Egan Beňová, T. - Viczenczová, C. - Soukup, Tomáš - Rauchová, Hana - Pavelka, Stanislav - Knezl, V. - Barančík, M. - Tribulová, N.
Cardiac Connexin-43 and PKC Signaling in Rats With Altered Thyroid Status Without and With Omega-3 Fatty Acids Intake
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Physiological Research. 2016, roč. 65, Suppl.1, S77-S90
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IF = 1.643
[ASEP]
Radošinská, J. - Kurahara, L. H. - Hiraishi, K. - Viczenczová, C. - Egan Beňová, T. - Szeiffová Bačová, B. - Dosenko, V. - Navarová, J. - Obšitník, B. - Imanaga, I. - Soukup, Tomáš - Tribulová, N.
Modulation of cardiac connexin-43 by omega-3 fatty acid ethyl-ester supplementation demonstrated in spontaneously diabetic rats
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Physiological Research. 2015, Vol. 64, 6, p. 795-806
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IF = 1.618
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Soukup, Tomáš - Diallo, Michael
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Proportions of myosin heavy chain mRNAs, protein isoforms and fiber types in the slow and fast skeletal muscles are maintained after alterations of thyroid status in rats
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Physiological Research. 2015, Vol. 64, 1, p. 111-118
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IF = 1.618
[ASEP]
Li, Y. - Adámek, Pavel - Zhang, H. - Tatsui, C. E. - Rhines, L. D. - Mrózková, Petra - Li, Q. - Kosturakis, A. K. - Cassidy, R. M. - Harrison, D. S. - Cata, J. P. - Sapire, K. - Zhang, H. - Kennamer-Chapman, R. M. - Jawad, A. B. - Ghetti, A. - Yan, J. - Paleček, Jiří - Dougherty, P. M.
The Cancer Chemotherapeutic Paclitaxel Increases Human and Rodent Sensory Neuron Responses to TRPV1 by Activation of TLR4
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Journal of Neuroscience. 2015, Vol. 35, 39, p. 13487-13500
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IF = 5.924
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Soukup, Tomáš - Smerdu, V.
Effect of altered innervation and thyroid hormones on myosin heavy chain expression and fiber type transitions: a mini-review
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Histochemistry and Cell Biology. 2015, Vol. 143, 2, p. 123-130
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IF = 2.78
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Uchytilová, Eva - Špicarová, Diana - Paleček, Jiří
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Single high-concentration capsaicin application prevents c-Fos expression in spinothalamic and postsynaptic dorsal column neurons after surgical incision
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European Journal of Pain. 2015, Vol. 19, 10, p. 1496-1505
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IF = 2.9
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Uchytilová, Eva - Špicarová, Diana - Paleček, Jiří
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TRPV1 antagonist attenuates postoperative hypersensitivity by central and peripheral mechanisms
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Molecular Pain 2014, roč. 10, 2014, p. 67. ISSN 1744-8069.
IF = 3.654
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An important approach in our research is recording of electrophysiological activity from dorsal horn neurons in acute spinal cord slices with patch-clamp technique. This method allows us recording of synaptic activity in the dorsal horn, where the nociceptive information between the peripheral and central nervous system is transmitted. The left image shows dorsal horn in spinal cord slice with a patch microelectrode at low magnification; on the right one a recorded neuron is visible under higher magnification.
For the study of cardiac and skeletal muscles histochemical and immunocytochemical staining of tissue sections is used. On the picture skeletal muscle sections stained for specific muscle proteins is shown enabling us quantitative analysis of different muscle fiber types (převzato z práce Soukup et al., Physiol Res 61: 575-586, 2012).
TRPV1 receptors are present on small diameter dorsal root ganglia neurons, that are involved in pain perception. Dorsal root ganglia neurons on histological slide stained for the presence of TRPV1 receptors (red) and parvalbumin (green).
